This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Held, M. R. Articles by Cappello, M. Search for Related Content PubMed PubMed Citation Articles by Held, M. R. Articles by Cappello, M.

Dietary Iron Content Mediates Hookworm Pathogenesis In Vivo

Program in International Child Health, Department of Pediatrics, Yale University School of Medicine, New Haven, Connecticut 06520,¹ Department of Animal and Avian Sciences and Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland 20742²

Received 12 September 2005/ Returned for modification 30 September 2005/ Accepted 18 October 2005

Hookworm infection is associated with growth delay and iron deficiency anemia in developing countries. A series of experiments were designed in order to test the hypothesis that host dietary iron restriction mediates susceptibility to hookworm infection using the hamster model of Ancylostoma ceylanicum. Animals were maintained on diets containing either 10 ppm iron (iron restricted) or 200 ppm iron (standard/high iron), followed by infection with A. ceylanicum third-stage larvae. Infected animals fed the standard diet exhibited statistically significant growth delay and reduced blood hemoglobin levels compared to uninfected controls on day 20 postinfection. In contrast, no statistically significant differences in weight or hemoglobin concentration were observed between infected and uninfected animals fed the iron-restricted diet. Moreover, iron-restricted animals were observed to have reduced intestinal worm burdens on day 10 and day 20 postinfection compared to those of animals maintained on the standard/high-iron diet. In a subsequent study, animals equilibrated on diets containing a range of iron levels (10 ppm, 40 ppm, 100 ppm, or 200 ppm) were infected with A. ceylanicum and followed for evidence of hookworm disease. Infected animals from the intermediate-dietary iron (40- and 100-ppm) groups exhibited greater weight loss and anemia than those in the low (10-ppm)- or high (200-ppm)-iron diet groups. Mortality was also significantly higher in the intermediate-dietary-iron groups. These data suggest that severe dietary iron restriction impairs hookworm development in vivo but that moderate iron restriction enhances host susceptibility to severe disease.

Editor: W. A. Petri, Jr.

This article has been cited by other articles:

• Dondji, B., Bungiro, R. D., Harrison, L. M., Vermeire, J. J., Bifulco, C., McMahon-Pratt, D., Cappello, M. (2008). Role for Nitric Oxide in Hookworm-Associated Immune Suppression. Infect. Immun. 76: 2560-2567 [Abstract] [Full Text]  
• Reiss, D., Harrison, L. M., Bungiro, R., Cappello, M. (2007). An Agar Plate Method for Culturing Hookworm Larvae: Analysis of Growth Kinetics and Infectivity Compared With Standard Coproculture Techniques. Am J Trop Med Hyg 77: 1087-1090 [Abstract] [Full Text]  
• Cappello, M., Bungiro, R. D., Harrison, L. M., Bischof, L. J., Griffitts, J. S., Barrows, B. D., Aroian, R. V. (2006). A purified Bacillus thuringiensis crystal protein with therapeutic activity against the hookworm parasite Ancylostoma ceylanicum. Proc. Natl. Acad. Sci. USA 103: 15154-15159 [Abstract] [Full Text]