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Infection and Immunity, January 2006, p. 305-312, Vol. 74, No. 1
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.1.305-312.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Identification of a Surface Protein of Streptococcus suis and Evaluation of Its Immunogenic and Protective Capacity in Pigs

Yuanyi Li,1 Gabriela Martinez,1 Marcelo Gottschalk,1 Sonia Lacouture,1 Philip Willson,2 J. Daniel Dubreuil,1 Mario Jacques,1 and Josee Harel1*

Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, C.P. 5000, St.-Hyacinthe, Québec J2S 7C6, Canada,1 Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, Saskatchewan S7N 5E3, Canada2

Received 8 June 2005/ Returned for modification 29 August 2005/ Accepted 22 September 2005

A Streptococcus suis surface protein reacting with convalescent-phase sera from pigs clinically infected by S. suis type 2 was identified. The apparent 110-kDa protein, designated Sao, exhibits typical features of membrane-anchored surface proteins of gram-positive bacteria, such as a signal sequence and an LPVTG membrane anchor motif. In spite of high identity with the partially sequenced genomes of S. suis Canadian strain 89/1591 and European strain P1/7, Sao does not share significant homology with other known sequences. However, a conserved avirulence domain that is often found in plant pathogens has been detected. Electron microscopy using an Sao-specific antiserum has confirmed the surface location of the Sao protein on S. suis. The Sao-specific antibody reacts with cell lysates of 28 of 33 S. suis serotypes and 25 of 26 serotype 2 isolates in immunoblots, suggesting its high conservation in S. suis species. The immunization of piglets with recombinant Sao elicits a significant humoral antibody response. However, the antibody response is not reflected in protection of pigs that are intratracheally challenged with a virulent strain in our conventional vaccination model.

* Corresponding author. Mailing address: Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, C.P. 5000, St.-Hyacinthe, Québec J2S 7C6, Canada. Phone: (450) 773-8521, ext. 18233. Fax: (450) 778-8108. E-mail: josee.harel{at}umontreal.ca.

Editor: J. D. Clements

Infection and Immunity, January 2006, p. 305-312, Vol. 74, No. 1
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.1.305-312.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Esgleas, M., Li, Y., Hancock, M. A., Harel, J., Dubreuil, J. D., Gottschalk, M. (2008). Isolation and characterization of {alpha}-enolase, a novel fibronectin-binding protein from Streptococcus suis. Microbiology 154: 2668-2679 [Abstract] [Full Text]  
  • Li, Y., Gottschalk, M., Esgleas, M., Lacouture, S., Dubreuil, J. D., Willson, P., Harel, J. (2007). Immunization with Recombinant Sao Protein Confers Protection against Streptococcus suis Infection. CVI 14: 937-943 [Abstract] [Full Text]  


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