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Infection and Immunity, January 2006, p. 528-536, Vol. 74, No. 1
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.1.528-536.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

CpG-Oligodeoxynucleotide Is a Potent Adjuvant with an Entamoeba histolytica Gal-Inhibitable Lectin Vaccine against Amoebic Liver Abscess in Gerbils

Catherine P. A. Ivory, Kathy Keller, and Kris Chadee*

Faculty of Medicine, Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, 3330 Hospital Dr. NW, Calgary, Alberta T2N 4N1, Canada

Received 16 August 2005/ Returned for modification 20 September 2005/ Accepted 30 September 2005

The protozoan parasite Entamoeba histolytica causes invasive amoebiasis characterized by amoebic dysentery and liver abscesses (ALA). The E. histolytica galactose/N-acetyl-D-galactosamine-inhibitable lectin (Gal-lectin), an immunogenic surface molecule involved in colonization and invasion, is a promising vaccine candidate against amoebiasis. Gal-lectin is known to induce Th1 cytokines in macrophages and spleen cells in vitro, and a Th1 response is thought to be protective against ALA. In this study, we report the use of cytosine guanine oligodeoxynucleotide (CpG-ODN) as adjuvant to augment Th1 responses against Gal-lectin in the gerbil model of ALA. Gerbils were vaccinated intramuscularly with the native Gal-lectin plus CpG-ODN or a paired non-CpG control GpC-ODN, and control gerbils received CpG-ODN alone. One week after the last boost gerbils were challenged intrahepatically with 106 amoebae. Gerbils receiving CpG-ODN as adjuvant with Gal-lectin were completely protected against the development of ALA, whereas 50% of gerbils receiving GpC-ODN and Gal-lectin developed ALA and 85% of controls developed ALA. Stronger lymphoproliferation in response to the Gal-lectin and higher prechallenge titers of serum Gal-lectin-specific antibodies, capable of blocking amoebic adherence, were observed when CpG-ODN was used as adjuvant. Gerbils vaccinated with CpG-ODN and Gal-lectin also had significantly higher levels of gamma interferon, interleukin-12 (IL-12), and IL-2 mRNA than controls. These data indicate that CpG-ODN can enhance the Th1 responses, which improve the protective effects of Gal-lectin. This is the first report of the use of CpG as a potent Th1 adjuvant with Gal-lectin to increase protection against ALA formation.

* Corresponding author. Mailing address: Faculty of Medicine, Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, 3330 Hospital Dr. NW, Calgary, Alberta T2N 4N1, Canada. Phone: (403) 210-3975. Fax: (403) 270-2772. E-mail:kchadee{at}uclagary.ca.

Editor: W. A. Petri, Jr.

Infection and Immunity, January 2006, p. 528-536, Vol. 74, No. 1
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.1.528-536.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ivory, C. P. A., Prystajecky, M., Jobin, C., Chadee, K. (2008). Toll-Like Receptor 9-Dependent Macrophage Activation by Entamoeba histolytica DNA. Infect. Immun. 76: 289-297 [Abstract] [Full Text]  
  • Ivory, C. P. A., Chadee, K. (2007). Intranasal Immunization with Gal-Inhibitable Lectin plus an Adjuvant of CpG Oligodeoxynucleotides Protects against Entamoeba histolytica Challenge. Infect. Immun. 75: 4917-4922 [Abstract] [Full Text]  
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