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Infection and Immunity, January 2006, p. 750-757, Vol. 74, No. 1
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.1.750-757.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Novel Three-Dimensional Organoid Model for Evaluation of the Interaction of Uropathogenic Escherichia coli with Terminally Differentiated Human Urothelial Cells

Yarery C. Smith,{dagger} Kerian K. Grande,{dagger} Susan B. Rasmussen, and Alison D. O'Brien*

Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814-4799

Received 18 May 2005/ Returned for modification 11 July 2005/ Accepted 25 October 2005

Human bladder 5637 cells cultivated under microgravity conditions formed organoids that displayed characteristics of in vivo tissue-specific differentiation. Uropathogenic Escherichia coli (UPEC) strain CP9 colonized and penetrated the organoids and induced {alpha}-hemolysin-mediated exfoliation of uroepithelial cells. We propose these uro-organoids as models that simulate the interactions between UPEC and terminally differentiated human urothelium.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Rm. B4052, 4301 Jones Bridge Rd., Bethesda, MD 20814-4799. Phone: (301) 295-3400. Fax: (301) 295-3773. E-mail: aobrien{at}usuhs.mil.

Editor: J. T. Barbieri

{dagger} Y. C. Smith and K. K. Grande contributed equally to this work.

Infection and Immunity, January 2006, p. 750-757, Vol. 74, No. 1
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.1.750-757.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Smith, Y. C., Rasmussen, S. B., Grande, K. K., Conran, R. M., O'Brien, A. D. (2008). Hemolysin of Uropathogenic Escherichia coli Evokes Extensive Shedding of the Uroepithelium and Hemorrhage in Bladder Tissue within the First 24 Hours after Intraurethral Inoculation of Mice. Infect. Immun. 76: 2978-2990 [Abstract] [Full Text]  
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