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Infection and Immunity, January 2006, p. 99-107, Vol. 74, No. 1
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.1.99-107.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Interaction of Cryptosporidium hominis and Cryptosporidium parvum with Primary Human and Bovine Intestinal Cells

Amna Hashim,1,2,3,4 Grace Mulcahy,3,4 Billy Bourke,1,2,4 and Marguerite Clyne1,2,4*

The Children's Research Centre, Our Lady's Hospital for Sick Children, Crumlin, Dublin 12, Ireland,1 UCD School of Medicine and Medical Science,2 UCD School of Agriculture, Food Science and Veterinary Medicine,3 UCD Conway Institute of Biomolecular and Biomedical Science, University College Dublin, Dublin 4, Ireland4

Received 6 July 2005/ Returned for modification 10 August 2005/ Accepted 30 September 2005

Cryptosporidiosis in humans is caused by the zoonotic pathogen Cryptosporidium parvum and the anthroponotic pathogen Cryptosporidium hominis. To what extent the recently recognized C. hominis species differs from C. parvum is unknown. In this study we compared the mechanisms of C. parvum and C. hominis invasion using a primary cell model of infection. Cultured primary bovine and human epithelial intestinal cells were infected with C. parvum or C. hominis. The effects of the carbohydrate lectin galactose-N-acetylgalactosamine (Gal/GalNAc) and inhibitors of cytoskeletal function and signal transduction mechanisms on entry of the parasites into host cells were tested. HCT-8 cells (human ileocecal adenocarcinoma cells) were used for the purpose of comparison. Pretreatment of parasites with Gal/GalNAc inhibited entry of C. parvum into HCT-8 cells and primary bovine cells but had no effect on entry of either C. parvum or C. hominis into primary human cells or on entry of C. hominis into HCT-8 cells. Both Cryptosporidium species entered primary cells by a protein kinase C (PKC)- and actin-dependent mechanism. Staurosporine, in particular, attenuated infection, likely through a combination of PKC inhibition and induction of apoptosis. Diversity in the mechanisms used by Cryptosporidium species to infect cells of different origins has important implications for understanding the relevance of in vitro studies of Cryptosporidium pathogenesis.

* Corresponding author. Mailing address: The Children's Research Centre, Our Lady's Hospital for Sick Children, Crumlin, Dublin 12, Ireland. Phone: 353 1 409 6959. Fax: 353 1 455 5307. E-mail: marguerite.clyne{at}ucd.ie.

Editor: W. A. Petri, Jr.

Infection and Immunity, January 2006, p. 99-107, Vol. 74, No. 1
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.1.99-107.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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  • Bhat, N., Joe, A., PereiraPerrin, M., Ward, H. D. (2007). Cryptosporidium p30, a Galactose/N-Acetylgalactosamine-specific Lectin, Mediates Infection in Vitro. J. Biol. Chem. 282: 34877-34887 [Abstract] [Full Text]  
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