This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Galioto, A. M. Articles by Abraham, D. Search for Related Content PubMed PubMed Citation Articles by Galioto, A. M. Articles by Abraham, D.

 Previous Article  |  Next Article 

Infection and Immunity, October 2006, p. 5730-5738, Vol. 74, No. 10
0019-9567/06/$08.00+0     doi:10.1128/IAI.01958-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Role of Eosinophils and Neutrophils in Innate and Adaptive Protective Immunity to Larval Strongyloides stercoralis in Mice

Department of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, Pennsylvania 19104,¹ Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104,² Department of Biochemistry and Molecular Biology, Mayo Clinic-Scottsdale, Scottsdale, Arizona 85259³

Received 1 December 2005/ Returned for modification 6 January 2006/ Accepted 3 July 2006

The goal of this study was to determine the roles of eosinophils and neutrophils in innate and adaptive protective immunity to larval Strongyloides stercoralis in mice. The experimental approach used was to treat mice with an anti-CCR3 monoclonal antibody to eliminate eosinophils or to use CXCR2^–/– mice, which have a severe neutrophil recruitment defect, and then determine the effect of the reduction or elimination of the particular cell type on larval killing. It was determined that eosinophils killed the S. stercoralis larvae in naïve mice, whereas these cells were not required for the accelerated killing of larvae in immunized mice. Experiments using CXCR2^–/– mice demonstrated that the reduction in recruitment of neutrophils resulted in significantly reduced innate and adaptive protective immunity. Protective antibody developed in the immunized CXCR2^–/– mice, thereby demonstrating that neutrophils were not required for the induction of the adaptive protective immune response. Moreover, transfer of neutrophil-enriched cell populations recovered from either wild-type or CXCR2^–/– mice into diffusion chambers containing larvae demonstrated that larval killing occurred with both cell populations when the diffusion chambers were implanted in immunized wild-type mice. Thus, the defect in the CXCR2^–/– mice was a defect in the recruitment of the neutrophils and not a defect in the ability of these cells to kill larvae. This study therefore demonstrated that both eosinophils and neutrophils are required in the protective innate immune response, whereas only neutrophils are necessary for the protective adaptive immune response to larval S. stercoralis in mice.

Editor: J. F. Urban, Jr.

This article has been cited by other articles:

• Wang, H.-B., Weller, P. F. (2008). Pivotal Advance: Eosinophils mediate early alum adjuvant-elicited B cell priming and IgM production. J. Leukoc. Biol. 83: 817-821 [Abstract] [Full Text]  
• Tateno, H., Li, H., Schur, M. J., Bovin, N., Crocker, P. R., Wakarchuk, W. W., Paulson, J. C. (2007). Distinct Endocytic Mechanisms of CD22 (Siglec-2) and Siglec-F Reflect Roles in Cell Signaling and Innate Immunity. Mol. Cell. Biol. 27: 5699-5710 [Abstract] [Full Text]  
• Padigel, U. M., Stein, L., Redding, K., Lee, J. J., Nolan, T. J., Schad, G. A., Birnbaumer, L., Abraham, D. (2007). Signaling through G{alpha}i2 protein is required for recruitment of neutrophils for antibody-mediated elimination of larval Strongyloides stercoralis in mice. J. Leukoc. Biol. 81: 1120-1126 [Abstract] [Full Text]