This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Galioto, A. M.
Right arrow Articles by Abraham, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Galioto, A. M.
Right arrow Articles by Abraham, D.

 Previous Article  |  Next Article 

Infection and Immunity, October 2006, p. 5730-5738, Vol. 74, No. 10
0019-9567/06/$08.00+0     doi:10.1128/IAI.01958-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Role of Eosinophils and Neutrophils in Innate and Adaptive Protective Immunity to Larval Strongyloides stercoralis in Mice

Ann Marie Galioto,1 Jessica A. Hess,1 Thomas J. Nolan,2 Gerhard A. Schad,2 James J. Lee,3 and David Abraham1*

Department of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, Pennsylvania 19104,1 Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104,2 Department of Biochemistry and Molecular Biology, Mayo Clinic-Scottsdale, Scottsdale, Arizona 852593

Received 1 December 2005/ Returned for modification 6 January 2006/ Accepted 3 July 2006

The goal of this study was to determine the roles of eosinophils and neutrophils in innate and adaptive protective immunity to larval Strongyloides stercoralis in mice. The experimental approach used was to treat mice with an anti-CCR3 monoclonal antibody to eliminate eosinophils or to use CXCR2–/– mice, which have a severe neutrophil recruitment defect, and then determine the effect of the reduction or elimination of the particular cell type on larval killing. It was determined that eosinophils killed the S. stercoralis larvae in naïve mice, whereas these cells were not required for the accelerated killing of larvae in immunized mice. Experiments using CXCR2–/– mice demonstrated that the reduction in recruitment of neutrophils resulted in significantly reduced innate and adaptive protective immunity. Protective antibody developed in the immunized CXCR2–/– mice, thereby demonstrating that neutrophils were not required for the induction of the adaptive protective immune response. Moreover, transfer of neutrophil-enriched cell populations recovered from either wild-type or CXCR2–/– mice into diffusion chambers containing larvae demonstrated that larval killing occurred with both cell populations when the diffusion chambers were implanted in immunized wild-type mice. Thus, the defect in the CXCR2–/– mice was a defect in the recruitment of the neutrophils and not a defect in the ability of these cells to kill larvae. This study therefore demonstrated that both eosinophils and neutrophils are required in the protective innate immune response, whereas only neutrophils are necessary for the protective adaptive immune response to larval S. stercoralis in mice.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Thomas Jefferson University, 233 S. 10th St., BLSB 530, Philadelphia, PA 19107. Phone: (215) 503-8917. Fax: (215) 923-9248. E-mail: david.abraham{at}jefferson.edu.

Editor: J. F. Urban, Jr.

Infection and Immunity, October 2006, p. 5730-5738, Vol. 74, No. 10
0019-9567/06/$08.00+0     doi:10.1128/IAI.01958-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Fabre, V., Beiting, D. P., Bliss, S. K., Gebreselassie, N. G., Gagliardo, L. F., Lee, N. A., Lee, J. J., Appleton, J. A. (2009). Eosinophil Deficiency Compromises Parasite Survival in Chronic Nematode Infection. J. Immunol. 182: 1577-1583 [Abstract] [Full Text]  
  • Wang, H.-B., Weller, P. F. (2008). Pivotal Advance: Eosinophils mediate early alum adjuvant-elicited B cell priming and IgM production. J. Leukoc. Biol. 83: 817-821 [Abstract] [Full Text]  
  • Tateno, H., Li, H., Schur, M. J., Bovin, N., Crocker, P. R., Wakarchuk, W. W., Paulson, J. C. (2007). Distinct Endocytic Mechanisms of CD22 (Siglec-2) and Siglec-F Reflect Roles in Cell Signaling and Innate Immunity. Mol. Cell. Biol. 27: 5699-5710 [Abstract] [Full Text]  
  • Padigel, U. M., Stein, L., Redding, K., Lee, J. J., Nolan, T. J., Schad, G. A., Birnbaumer, L., Abraham, D. (2007). Signaling through G{alpha}i2 protein is required for recruitment of neutrophils for antibody-mediated elimination of larval Strongyloides stercoralis in mice. J. Leukoc. Biol. 81: 1120-1126 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»