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Infection and Immunity, October 2006, p. 5834-5839, Vol. 74, No. 10
0019-9567/06/$08.00+0     doi:10.1128/IAI.00438-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Transcutaneous Immunization with Toxin-Coregulated Pilin A Induces Protective Immunity against Vibrio cholerae O1 El Tor Challenge in Mice

Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts,¹ Department of Medicine, Harvard Medical School, Boston, Massachusetts,² Dartmouth Medical School, Hanover, New Hampshire,³ Department of Pediatrics, Harvard Medical School, Boston, Massachusetts,⁴ ICDDR,B Centre for Health and Populations Studies, Dhaka, Bangladesh,⁵ Department of Microbiology and Molecular Center, Harvard Medical School, Boston, Massachusetts,⁶ Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts⁷

Received 17 March 2006/ Returned for modification 2 June 2006/ Accepted 26 July 2006

Toxin-coregulated pilin A (TcpA) is the main structural subunit of a type IV bundle-forming pilus of Vibrio cholerae, the cause of cholera. Toxin-coregulated pilus is involved in formation of microcolonies of V. cholerae at the intestinal surface, and strains of V. cholerae deficient in TcpA are attenuated and unable to colonize intestinal surfaces. Anti-TcpA immunity is common in humans recovering from cholera in Bangladesh, and immunization against TcpA is protective in murine V. cholerae models. To evaluate whether transcutaneously applied TcpA is immunogenic, we transcutaneously immunized mice with 100 µg of TcpA or TcpA with an immunoadjuvant (cholera toxin [CT], 50 µg) on days 0, 19, and 40. Mice immunized with TcpA alone did not develop anti-TcpA responses. Mice that received transcutaneously applied TcpA and CT developed prominent anti-TcpA immunoglobulin G (IgG) serum responses but minimal anti-TcpA IgA. Transcutaneous immunization with CT induced prominent IgG and IgA anti-CT serum responses. In an infant mouse model, offspring born to dams transcutaneously immunized either with TcpA and CT or with CT alone were challenged with 10⁶ CFU (one 50% lethal dose) wild-type V. cholerae O1 El Tor strain N16961. At 48 h, mice born to females transcutaneously immunized with CT alone had 36% ± 10% (mean ± standard error of the mean) survival, while mice born to females transcutaneously immunized with TcpA and CT had 69% ± 6% survival (P < 0.001). Our results suggest that transcutaneous immunization with TcpA and an immunoadjuvant induces protective anti-TcpA immune responses. Anti-TcpA responses may contribute to an optimal cholera vaccine.

Editor: V. J. DiRita

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