This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by O'Grady, E. P.
Right arrow Articles by O'Gara, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by O'Grady, E. P.
Right arrow Articles by O'Gara, F.

 Previous Article  |  Next Article 

Infection and Immunity, October 2006, p. 5893-5902, Vol. 74, No. 10
0019-9567/06/$08.00+0     doi:10.1128/IAI.00489-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Pseudomonas aeruginosa Infection of Airway Epithelial Cells Modulates Expression of Kruppel-Like Factors 2 and 6 via RsmA-Mediated Regulation of Type III Exoenzymes S and Y{dagger}

Eoin P. O'Grady, Heidi Mulcahy, Julie O'Callaghan, Claire Adams, and Fergal O'Gara*

BIOMERIT Research Centre, Department of Microbiology, University College Cork, Cork, Ireland

Received 25 March 2006/ Returned for modification 30 May 2006/ Accepted 12 July 2006

Pseudomonas aeruginosa is an important opportunistic pathogen which is capable of causing both acute and chronic infections in immunocompromised patients. Successful adaptation of the bacterium to its host environment relies on the ability of the organism to tightly regulate gene expression. RsmA, a small RNA-binding protein, controls the expression of a large number of virulence-related genes in P. aeruginosa, including those encoding the type III secretion system and associated effector proteins, with important consequences for epithelial cell morphology and cytotoxicity. In order to examine the influence of RsmA-regulated functions in the pathogen on gene expression in the host, we compared global expression profiles of airway epithelial cells in response to infection with P. aeruginosa PAO1 and an rsmA mutant. The RsmA-dependent response of host cells was characterized by significant changes in the global transcriptional pattern, including the increased expression of two Kruppel-like factors, KLF2 and KLF6. This increased expression was mediated by specific type III effector proteins. ExoS was required for the enhanced expression of KLF2, whereas both ExoS and ExoY were required for the enhanced expression of KLF6. Neither ExoT nor ExoU influenced the expression of the transcription factors. Additionally, the increased gene expression of KLF2 and KLF6 was associated with ExoS-mediated cytotoxicity. Therefore, this study identifies for the first time the human transcription factors KLF2 and KLF6 as targets of the P. aeruginosa type III exoenzymes S and Y, with potential importance in host cell death.

* Corresponding author. Mailing address: BIOMERIT Research Centre, Department of Microbiology, University College Cork, Cork, Ireland. Phone: 353-21-4901315. Fax: 353-21-4275934. E-mail: f.ogara{at}ucc.ie.

{dagger} Supplemental material for this article may be found at http://iai.asm.org/.

Editor: V. J. DiRita

Infection and Immunity, October 2006, p. 5893-5902, Vol. 74, No. 10
0019-9567/06/$08.00+0     doi:10.1128/IAI.00489-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Dach, K., Zovko, J., Hogardt, M., Koch, I., van Erp, K., Heesemann, J., Hoffmann, R. (2009). Bacterial Toxins Induce Sustained mRNA Expression of the Silencing Transcription Factor klf2 via Inactivation of RhoA and Rhophilin 1. Infect. Immun. 77: 5583-5592 [Abstract] [Full Text]  
  • Kiely, P. D., O'Callaghan, J., Abbas, A., O'Gara, F. (2008). Genetic analysis of genes involved in dipeptide metabolism and cytotoxicity in Pseudomonas aeruginosa PAO1. Microbiology 154: 2209-2218 [Abstract] [Full Text]  
  • Fields, J. A., Thompson, S. A. (2008). Campylobacter jejuni CsrA Mediates Oxidative Stress Responses, Biofilm Formation, and Host Cell Invasion. J. Bacteriol. 190: 3411-3416 [Abstract] [Full Text]  
  • Mulcahy, H., O'Callaghan, J., O'Grady, E. P., Macia, M. D., Borrell, N., Gomez, C., Casey, P. G., Hill, C., Adams, C., Gahan, C. G. M., Oliver, A., O'Gara, F. (2008). Pseudomonas aeruginosa RsmA Plays an Important Role during Murine Infection by Influencing Colonization, Virulence, Persistence, and Pulmonary Inflammation. Infect. Immun. 76: 632-638 [Abstract] [Full Text]  
  • Soscia, C., Hachani, A., Bernadac, A., Filloux, A., Bleves, S. (2007). Cross Talk between Type III Secretion and Flagellar Assembly Systems in Pseudomonas aeruginosa. J. Bacteriol. 189: 3124-3132 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»