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Infection and Immunity, October 2006, p. 5989-6000, Vol. 74, No. 10
0019-9567/06/$08.00+0     doi:10.1128/IAI.00138-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Escherichia coli Prevents Phagocytosis-Induced Death of Macrophages via Classical NF-B Signaling, a Link to T-Cell Activation

Heinrich V. Groesdonk, Silke Schlottmann, Friederike Richter, Michael Georgieff, and Uwe Senftleben^*

Department of Anesthesiology and Intensive Care, University of Ulm, Ulm, Germany

Received 26 January 2006/ Returned for modification 30 March 2006/ Accepted 20 July 2006

NF-B is a crucial mediator of macrophage inflammatory responses, but its role in the context of pathogen-induced adaptive immune responses has yet to be elucidated. Here, we demonstrate that classical NF-B activation delays phagocytosis-induced cell death (PICD) in Raw 264.7 and bone marrow-derived macrophages (BMDMs) upon ingestion of bacteria from the Escherichia coli laboratory strain Top10. By expression of a nondegradable form of IB (superrepressor) and pyrrolidine dithiocarbamate treatment, prolonged activation of NF-B upon bacterial coculture is suppressed, whereas initial induction is only partially inhibited. This activation pattern results in partial inhibition of cellular activation and reduced expression of costimulatory CD86. Notably, suppression of classical NF-B activation does not influence bacterial uptake rates but is followed by increased production of oxygen radicals and enhanced intracellular killing in Raw macrophages. This is associated with reduced expression of NF-B-dependent antiapoptotic c-IAP-2 and a loss of the mitochondrial transmembrane potential. Accordingly, NF-B inhibition in Raw cells and BMDMs causes increased apoptotic rates within 12 h of bacterial ingestion. Interestingly, accelerated eradication of E. coli in NF-B-inhibited macrophages is associated with reduced antigen-specific T-cell activation in macrophage-lymphocyte cocultures. These data suggest that E. coli inhibits PICD of macrophages via classical, antiapoptotic NF-B activation and thus facilitates signaling to T cells. Subsequently, a proper adaptive immune response is likely to be generated. Conclusively, therapeutic inhibition of classical NF-B activation in macrophages may hamper the initiation of adaptive immunity.

Editor: F. C. Fang

Heinrich V. Groesdonk and Silke Schlottmann contributed equally to this work.

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