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Infection and Immunity, October 2006, p. 6001-6005, Vol. 74, No. 10
0019-9567/06/$08.00+0     doi:10.1128/IAI.00512-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Overexpression, Purification, Characterization, and Pathogenicity of Vibrio harveyi Hemolysin VHH

Yingbin Zhong,1 Xiao-Hua Zhang,1* Jixiang Chen,1 Zhenghao Chi,1 Boguang Sun,1 Yun Li,1 and Brian Austin2

Department of Marine Biology, Ocean University of China, Qingdao, China,1 School of Life Sciences, John Muir Building, Heriot-Watt University, Riccarton, Edinburgh, EH14 4AS, United Kingdom2

Received 29 March 2006/ Returned for modification 25 May 2006/ Accepted 12 July 2006

Vibrio harveyi VHH hemolysin is a putative pathogenicity factor in fish. In this study, the hemolysin gene vhhA was overexpressed in Escherichia coli, and the purified VHH was characterized with regard to pH and temperature profiles, phospholipase activity, cytotoxicity, pathogenicity to flounder, and the signal peptide.

* Corresponding author. Mailing address: Department of Marine Biology, Ocean University of China, 5 Yushan Road, Qingdao 266003, People's Republic of China. Phone and fax: 0086-532-82032266. E-mail: xhzhang{at}ouc.edu.cn.

Editor: V. J. DiRita

Infection and Immunity, October 2006, p. 6001-6005, Vol. 74, No. 10
0019-9567/06/$08.00+0     doi:10.1128/IAI.00512-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sun, B., Zhang, X.-H., Tang, X., Wang, S., Zhong, Y., Chen, J., Austin, B. (2007). A Single Residue Change in Vibrio harveyi Hemolysin Results in the Loss of Phospholipase and Hemolytic Activities and Pathogenicity for Turbot (Scophthalmus maximus). J. Bacteriol. 189: 2575-2579 [Abstract] [Full Text]  


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