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Infection and Immunity, October 2006, p. 6006-6010, Vol. 74, No. 10
0019-9567/06/$08.00+0     doi:10.1128/IAI.00545-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Application of Mutated Clostridium difficile Toxin A for Determination of Glucosyltransferase-Dependent Effects

Matthias Teichert, Helma Tatge, Janett Schoentaube, Ingo Just, and Ralf Gerhard*

Institute of Toxicology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany

Received 4 April 2006/ Returned for modification 15 May 2006/ Accepted 16 July 2006

Mutation of tryptophan-101 in Clostridium difficile toxin A, a 308-kDa glucosyltransferase, resulted in a 50-fold-reduced cytopathic activity in cell culture experiments. The mutant toxin A was characterized and applied to distinguish between glucosyltransferase-dependent and -independent effects with respect to RhoB up-regulation as a cellular stress response.

* Corresponding author. Mailing address: Institut für Toxikologie, Medizinische Hochschule Hannover, Carl-Neuberg-Str. 1, 30625 Hannover, Germany. Phone: 49 511 532 2810. Fax: 49 511 532 2879. E-mail: gerhard.ralf{at}mh-hannover.de.

Editor: J. T. Barbieri

Infection and Immunity, October 2006, p. 6006-6010, Vol. 74, No. 10
0019-9567/06/$08.00+0     doi:10.1128/IAI.00545-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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