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Infection and Immunity, November 2006, p. 6293-6299, Vol. 74, No. 11
0019-9567/06/$08.00+0     doi:10.1128/IAI.00068-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Host-Derived Pentapeptide Affecting Adhesion, Proliferation, and Local pH in Biofilm Communities Composed of Streptococcus and Actinomyces Species{triangledown}

Mirva Drobni,1,{dagger} Tong Li,1,{dagger} Carina Krüger,2 Vuokko Loimaranta,1 Mogens Kilian,3 Lennart Hammarström,2 Hans Jörnvall,4 Tomas Bergman,4 and Nicklas Strömberg1*

Department of Odontology/Cariology, Umeå University, SE-901 87 Umeå, Sweden,1 Division of Clinical Immunology, Karolinska University Hospital Huddinge, SE-141 86 Stockholm, Sweden,2 Department of Medical Microbiology and Immunology, Aarhus University, DK-8000 Aarhus C, Denmark,3 Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77, Stockholm, Sweden4

Received 13 January 2006/ Returned for modification 7 April 2006/ Accepted 15 August 2006

Salivary proline-rich proteins (PRPs) attach commensal Actinomyces and Streptococcus species to teeth. Here, gel filtration, mass spectrometry and Edman degradation were applied to show the release of a pentapeptide, RGRPQ, from PRP-1 upon proteolysis by Streptococcus gordonii. Moreover, synthetic RGRPQ and derivatives were used to investigate associated innate properties and responsible motifs. The RGRPQ peptide increased 2.5-fold the growth rate of S. gordonii via a Q-dependent sequence motif and selectively stimulated oral colonization of this organism in a rat model in vivo. In contrast, the growth of Streptococcus mutans, implicated in caries, was not affected. While the entire RGRPQ sequence was required to block sucrose-induced pH-decrease by S. gordonii and S. mutans, the N-terminal Arg residue mediated the pH increase (i.e., ammonia production) by S. gordonii alone (which exhibits Arg catabolism to ammonia). Strains of commensal viridans streptococci exhibited PRP degradation and Arg catabolism, whereas cariogenic species did not. The RGRPQ peptide mediated via a differential Q-dependent sequence motif, adhesion inhibition, and desorption of PRP-1-binding strains of A. naeslundii genospecies 2 (5 of 10 strains) but not of S. gordonii (n = 5). The inhibitable A. naeslundii strains alone displayed the same binding profile as S. gordonii to hybrid peptides terminating in RGRPQ or GQSPQ, derived from the middle or C-terminal segments of PRP-1. The present findings indicate the presence of a host-bacterium interaction in which a host peptide released by bacterial proteolysis affects key properties in biofilm formation.

* Corresponding author. Mailing address: Department of Odontology/Cariology, Umeå University, SE-901 87 Umeå, Sweden. Phone: 46 90 7856030. Fax: 46 90 770580. E-mail: Nicklas.Stromberg{at}odont.umu.se.

{triangledown} Published ahead of print on 28 August 2006.

Editor: V. J. DiRita

{dagger} M.D. and T.L. contributed equally to this study.

Infection and Immunity, November 2006, p. 6293-6299, Vol. 74, No. 11
0019-9567/06/$08.00+0     doi:10.1128/IAI.00068-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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