This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bonnett, C. R.
Right arrow Articles by Burritt, J. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bonnett, C. R.
Right arrow Articles by Burritt, J. B.

 Previous Article  |  Next Article 

Infection and Immunity, December 2006, p. 6528-6539, Vol. 74, No. 12
0019-9567/06/$08.00+0     doi:10.1128/IAI.00909-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Early Neutrophil Recruitment and Aggregation in the Murine Lung Inhibit Germination of Aspergillus fumigatus Conidia{triangledown}

Colin R. Bonnett,1,{dagger} E. Jean Cornish,1 Allen G. Harmsen,2 and James B. Burritt1*

Departments of Microbiology,1 Veterinary Molecular Biology, Montana State University, Bozeman, Montana 597172

Received 7 June 2006/ Returned for modification 1 July 2006/ Accepted 7 August 2006

Several types of polymorphonuclear neutrophil (PMN) deficiency are a predisposing condition for fatal Aspergillus fumigatus infection. In order to study the defensive role of PMNs in the lungs, with particular reference to PMN recruitment and antimicrobial oxidant activity, responses to pulmonary instillation of A. fumigatus conidia were examined. Responses in BALB/c and C57BL/6 mice were compared with those in CXCR2–/– and gp91phox–/– mice, which are known to have delayed recruitment of PMN to the lungs in response to inflammatory stimuli and inactive NADPH oxidase, respectively. In BALB/c mice, PMNs were recruited to the lungs and formed oxidase-active aggregates with conidia, which inhibited germination. In C57BL/6, gp91phox–/–, and CXCR2–/– mice, PMN recruitment was slower and there was increased germination compared to that in BALB/c mice at 6 and 12 h. In gp91phox–/– mice, germination was extensive in PMN aggregates but negligible in alveolar macrophages (AM). Lung sections taken at 6 and 48 h from BALB/c mice showed PMN accumulation at peribronchiolar sites but no germinating conidia. Those from C57BL/6 and CXCR2–/– mice showed germinating conidia at 6 h but not at 48 h and few inflammatory cells. In contrast, those from gp91phox–/– mice showed germination at 6 h with more-extensive hyphal proliferation and tissue invasion at 48 h. These results indicate that when the lungs are exposed to large numbers of conidia, in addition to the phagocytic activity of AM, early PMN recruitment and formation of oxidative-active aggregates are essential in preventing germination of A. fumigatus conidia.

* Corresponding author. Mailing address: 109 Lewis Hall, Department of Microbiology, Montana State University, Bozeman, MT 59717. Phone: (406) 994-5667. Fax: (406) 994-4926. E-mail: jburritt{at}montana.edu.

{triangledown} Published ahead of print on 18 August 2006.

Editor: A. Casadevall

{dagger} Present address: College of Veterinary Medicine, Washington State University, PO Box 647012, Pullman, WA 99164-7012.

Infection and Immunity, December 2006, p. 6528-6539, Vol. 74, No. 12
0019-9567/06/$08.00+0     doi:10.1128/IAI.00909-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Park, S. J., Mehrad, B. (2009). Innate Immunity to Aspergillus Species. Clin. Microbiol. Rev. 22: 535-551 [Abstract] [Full Text]  
  • Bianchi, M., Hakkim, A., Brinkmann, V., Siler, U., Seger, R. A., Zychlinsky, A., Reichenbach, J. (2009). Restoration of NET formation by gene therapy in CGD controls aspergillosis. Blood 114: 2619-2622 [Abstract] [Full Text]  
  • Dagenais, T. R. T., Keller, N. P. (2009). Pathogenesis of Aspergillus fumigatus in Invasive Aspergillosis. Clin. Microbiol. Rev. 22: 447-465 [Abstract] [Full Text]  
  • Hu, J., Wang, Y., Xie, L. (2009). Potential Role of Macrophages in Experimental Keratomycosis. IOVS 50: 2087-2094 [Abstract] [Full Text]  
  • Werner, J. L., Metz, A. E., Horn, D., Schoeb, T. R., Hewitt, M. M., Schwiebert, L. M., Faro-Trindade, I., Brown, G. D., Steele, C. (2009). Requisite Role for the Dectin-1 {beta}-Glucan Receptor in Pulmonary Defense against Aspergillus fumigatus. J. Immunol. 182: 4938-4946 [Abstract] [Full Text]  
  • Cornish, E. J., Hurtgen, B. J., McInnerney, K., Burritt, N. L., Taylor, R. M., Jarvis, J. N., Wang, S. Y., Burritt, J. B. (2008). Reduced Nicotinamide Adenine Dinucleotide Phosphate Oxidase-Independent Resistance to Aspergillus fumigatus in Alveolar Macrophages. J. Immunol. 180: 6854-6867 [Abstract] [Full Text]  
  • Meissner, N., Rutkowski, M., Harmsen, A. L., Han, S., Harmsen, A. G. (2007). Type I Interferon Signaling and B Cells Maintain Hemopoiesis during Pneumocystis Infection of the Lung. J. Immunol. 178: 6604-6615 [Abstract] [Full Text]  
  • Feldmesser, M. (2006). Role of Neutrophils in Invasive Aspergillosis{triangledown}. Infect. Immun. 74: 6514-6516 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»