This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Whary, M. T. Articles by Fox, J. G. Search for Related Content PubMed PubMed Citation Articles by Whary, M. T. Articles by Fox, J. G.

 Previous Article  |  Next Article 

Infection and Immunity, December 2006, p. 6615-6623, Vol. 74, No. 12
0019-9567/06/$08.00+0     doi:10.1128/IAI.01091-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Rapid Onset of Ulcerative Typhlocolitis in B6.129P2-IL10^tm1Cgn (IL-10^–/–) Mice Infected with Helicobacter trogontum Is Associated with Decreased Colonization by Altered Schaedler's Flora

M. T. Whary,^1* S. J. Danon,² Y. Feng,¹ Z. Ge,¹ N. Sundina,¹ V. Ng,¹ N. S. Taylor,¹ A. B. Rogers,¹ and J. G. Fox¹

Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139,¹ CSIRO Molecular & Health Technologies, North Ryde, New South Wales, Australia 1670²

Received 12 July 2006/ Returned for modification 28 August 2006/ Accepted 12 September 2006

Infection with Helicobacter trogontum, a urease-positive helicobacter isolated from subclinically infected rats, was evaluated in B6.129P2-IL10^tm1Cgn (interleukin-10^–/– [IL-10^–/–]) and C57BL/6 (B6) mice. In a first experiment, IL-10^–/– mice naturally infected with Helicobacter rodentium had subclinical typhlocolitis but developed severe diarrhea and loss of body condition with erosive to ulcerative typhlocolitis within 1 to 3 weeks of experimental infection with H. trogontum. A second experiment demonstrated that helicobacter-free IL-10^–/– mice dosed with H. trogontum also developed severe clinical signs and typhlocolitis within 2 to 4 weeks, whereas B6 mice colonized with H. trogontum were resistant to disease. In a third experiment, using helicobacter-free IL-10^–/– mice, dosing with H. trogontum resulted in acute morbidity and typhlocolitis within 8 days. Acute typhlocolitis was accompanied by signs of sepsis supported by degenerative hemograms and recovery of Escherichia coli and Proteus spp. from the livers of infected mice. Quantitative PCR data revealed that H. rodentium and H. trogontum may compete for colonization of the lower bowel, as H. trogontum established higher colonization levels in the absence of H. rodentium (P < 0.003). H. trogontum-induced typhlocolitis was also associated with a significant decrease in the levels of colonization by five of eight anaerobes that comprise altered Schaedler's flora (P < 0.002). These results demonstrate for the first time that H. rodentium infection in IL-10^–/– mice causes subclinical typhlocolitis and that infection with H. trogontum (with or without H. rodentium) induces a rapid-onset, erosive to ulcerative typhlocolitis which impacts the normal anaerobic flora of the colon and increases the risk of sepsis.

---

* Corresponding author. Mailing address: 77 Massachusetts Ave., Bldg. 16-825A, Cambridge, MA 02139. Phone: (617) 253-9435. Fax: (617) 258-5708. E-mail: mwhary{at}mit.edu.

Published ahead of print on 18 September 2006.

Editor: A. D. O'Brien

---

Infection and Immunity, December 2006, p. 6615-6623, Vol. 74, No. 12
0019-9567/06/$08.00+0     doi:10.1128/IAI.01091-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Shen, Z., Feng, Y., Rogers, A. B., Rickman, B., Whary, M. T., Xu, S., Clapp, K. M., Boutin, S. R., Fox, J. G. (2009). Cytolethal Distending Toxin Promotes Helicobacter cinaedi-Associated Typhlocolitis in Interleukin-10-Deficient Mice. Infect. Immun. 77: 2508-2516 [Abstract] [Full Text]  
• Fox, J G (2007). Helicobacter bilis: bacterial provocateur orchestrates host immune responses to commensal flora in a model of inflammatory bowel disease. Gut 56: 898-900 [Full Text]