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Infection and Immunity, December 2006, p. 6778-6784, Vol. 74, No. 12
0019-9567/06/$08.00+0     doi:10.1128/IAI.01187-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Limited Cross-Reactivity among Domains of the Plasmodium falciparum Clone 3D7 Erythrocyte Membrane Protein 1 Family{triangledown}

Louise Joergensen,1 Louise Turner,1 Pamela Magistrado,1 Madeleine A. Dahlbäck,1 Lasse S. Vestergaard,1 John P. Lusingu,1,2 Martha Lemnge,2 Ali Salanti,1 Thor G. Theander,1 and Anja T. R. Jensen1*

Centre for Medical Parasitology at Department of Medical Microbiology and Immunology, University of Copenhagen and Copenhagen University Hospital, 2200 Copenhagen, Denmark,1 National Institute for Medical Research, NIMR Tanga Centre, P.O. Box 5004, Tanga, Tanzania2

Received 28 July 2006/ Returned for modification 5 September 2006/ Accepted 20 September 2006

The var gene-encoded Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family is responsible for antigenic variation and sequestration of infected erythrocytes during malaria. We have previously grouped the 60 PfEMP1 variants of P. falciparum clone 3D7 into groups A and B/A (category A) and groups B, B/C, and C (category non-A). Expression of category A molecules is associated with severe malaria, and that of category non-A molecules is associated with uncomplicated malaria and asymptomatic infection. Here we assessed cross-reactivity among 60 different recombinant PfEMP1 domains derived from clone 3D7 by using a competition enzyme-linked immunosorbent assay and a pool of plasma from 63 malaria-exposed Tanzanian individuals. We conclude that naturally acquired antibodies are largely directed toward epitopes varying between different domains with a few, mainly category A, domains sharing cross-reactive antibody epitopes. Identification of groups of serological cross-reacting molecules is pivotal for the development of vaccines based on PfEMP1.

* Corresponding author. Mailing address: University of Copenhagen, Department of Medical Microbiology and Immunology, Panum 24.2.24, Blegdamsvej 3, Copenhagen DK-2200, Denmark. Phone: 4535327682. Fax: 4535327851. E-mail: atrj{at}cmp.dk.

{triangledown} Published ahead of print on 2 October 2006.

Editor: W. A. Petri, Jr.

Infection and Immunity, December 2006, p. 6778-6784, Vol. 74, No. 12
0019-9567/06/$08.00+0     doi:10.1128/IAI.01187-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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