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Infection and Immunity, December 2006, p. 6806-6810, Vol. 74, No. 12
0019-9567/06/$08.00+0     doi:10.1128/IAI.01210-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Microneedle-Based Intradermal Delivery of the Anthrax Recombinant Protective Antigen Vaccine{triangledown}

John A. Mikszta,1* John P. Dekker III,1 Noel G. Harvey,1 Cheryl H. Dean,1 John M. Brittingham,1 Joanne Huang,1 Vincent J. Sullivan,1 Beverly Dyas,2 Chad J. Roy,2,{dagger} and Robert G. Ulrich2

BD Technologies, Research Triangle Park, North Carolina 27709,1 U.S. Army Medical Research Institute of Infectious Diseases, Frederick, Maryland 217022

Received 1 August 2006/ Returned for modification 15 September 2006/ Accepted 21 September 2006

The recombinant protective antigen (rPA) of Bacillus anthracis is a promising anthrax vaccine. We compared serum immunoglobulin G levels and toxin-neutralizing antibody titers in rabbits following delivery of various doses of vaccine by microneedle-based intradermal (i.d.) delivery or intramuscular (i.m.) injection using conventional needles. Intradermal delivery required less antigen to induce levels of antibody similar to those produced via i.m. injection during the first 2 weeks following primary and booster inoculation. This dose-sparing effect was less evident at the later stages of the immune response. Rabbits immunized i.d. with 10 µg of rPA displayed 100% protection from aerosol spore challenge, while i.m. injection of the same dose provided slightly lower protection (71%). Groups immunized with lower antigen doses were partially protected (13 to 29%) regardless of the mode of administration. Overall, our results suggest rPA formulated with aluminum adjuvant and administered to the skin by a microneedle-based device is as efficacious as i.m. vaccination.

* Corresponding author. Mailing address: BD Technologies, 21 Davis Drive, Research Triangle Park, NC 27709. Phone: (919) 597-6158. Fax: (919) 597-6402. E-mail: john_mikszta{at}bd.com.

{triangledown} Published ahead of print on 9 October 2006.

Editor: D. L. Burns

{dagger} Present address: Tulane National Primate Research Center, Division of Microbiology, Covington, LA 70433.

Infection and Immunity, December 2006, p. 6806-6810, Vol. 74, No. 12
0019-9567/06/$08.00+0     doi:10.1128/IAI.01210-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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