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Infection and Immunity, December 2006, p. 6811-6820, Vol. 74, No. 12
0019-9567/06/$08.00+0     doi:10.1128/IAI.01188-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Helicobacter pylori HP1512 Is a Nickel-Responsive NikR-Regulated Outer Membrane Protein{triangledown}

Gregg S. Davis, Erika L. Flannery, and Harry L. T. Mobley*

Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan

Received 28 July 2006/ Returned for modification 31 August 2006/ Accepted 21 September 2006

Helicobacter pylori is dependent upon the production of the highly abundant and active metalloenzyme urease for colonization of the human stomach. Thus, H. pylori has an absolute requirement for the transition metal nickel, a required cofactor for urease. To investigate the contribution of genes that are factors in this process, microarray analysis comparing the transcriptome of wild-type H. pylori 26695 cultured in brucella broth containing fetal calf serum (BBF) alone or supplemented with 100 µM NiCl2 suggested that HP1512 is repressed in the presence of 100 µM supplemental nickel. When measured by comparative real-time quantitative PCR (qPCR), HP1512 transcription was reduced 43-fold relative to the value for the wild type when cultured in BBF supplemented with 10 µM NiCl2. When grown in unsupplemented BBF, urease activity of an HP1512::cat mutant was significantly reduced compared to the wild type, 4.9 ± 0.5 µmol/min/mg of protein (n = 7) and 17.1 ± 4.9 µmol/min/mg of protein (n = 13), respectively (P < 0.0001). In silico analysis of the HP1511-HP1512 (HP1511-1512) intergenic region identified a putative NikR operator upstream of HP1512. Gel shift analysis with purified recombinant NikR verified nickel-dependent binding of H. pylori NikR to the HP1511-1512 intergenic region. Furthermore, comparative real-time qPCR of four nickel-related genes suggests that mutation of HP1512 results in reduced intracellular nickel concentration relative to wild-type H. pylori 26695. Taken together, these data suggest that HP1512 encodes a NikR-nickel-regulated outer membrane protein.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Michigan Medical School, 1150 W. Medical Center Dr., 5641 Medical Sciences II, Ann Arbor, MI 48109-0620. Phone: (734) 763-3531. Fax: (734) 764-3562. E-mail: hmobley{at}umich.edu.

{triangledown} Published ahead of print on 9 October 2006.

Editor: J. N. Weiser

Infection and Immunity, December 2006, p. 6811-6820, Vol. 74, No. 12
0019-9567/06/$08.00+0     doi:10.1128/IAI.01188-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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