This Article Full Text Full Text (PDF) Other Versions of this Article: IAI.00648-06v1 74/12/6965    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Deiwick, J. Articles by Méresse, S. Search for Related Content PubMed PubMed Citation Articles by Deiwick, J. Articles by Méresse, S.

 Previous Article  |  Next Article 

Infection and Immunity, December 2006, p. 6965-6972, Vol. 74, No. 12
0019-9567/06/$08.00+0     doi:10.1128/IAI.00648-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The Translocated Salmonella Effector Proteins SseF and SseG Interact and Are Required To Establish an Intracellular Replication Niche

Jörg Deiwick,^1,3*, Suzana P. Salcedo,^2, Emmanuel Boucrot,¹ Sarah M. Gilliland,² Thomas Henry,¹ Nele Petermann,³ Scott R. Waterman,² Jean-Pierre Gorvel,¹ David W. Holden,^2, and Stéphane Méresse^1,

Centre d'Immunologie de Marseille-Luminy, CNRS-INSERM-Université de la Méditerranée, Parc Scientifique de Luminy, Case 906-13288, Marseille Cedex 9, France,¹ Department of Infectious Diseases, Centre for Molecular Microbiology and Infection, Imperial College London, Armstrong Road, London SW7 2AZ, United Kingdom,² Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany³

Received 21 April 2006/ Returned for modification 30 May 2006/ Accepted 6 September 2006

The facultative intracellular pathogen Salmonella enterica causes a variety of diseases, including gastroenteritis and typhoid fever. Inside epithelial cells, Salmonella replicates in vacuoles, which localize in the perinuclear area in close proximity to the Golgi apparatus. Among the effector proteins translocated by the Salmonella pathogenicity island 2-encoded type III secretion system, SifA and SseG have been shown necessary but not sufficient to ensure the intracellular positioning of Salmonella vacuoles. Hence, we have investigated the involvement of other secreted effector proteins in this process. Here we show that SseF interacts functionally and physically with SseG but not SifA and is also required for the perinuclear localization of Salmonella vacuoles. The observations show that the intracellular positioning of Salmonella vacuoles is a complex phenomenon resulting from the combined action of several effector proteins.

Published ahead of print on 2 October 2006.

Editor: F. C. Fang

J.D. and S.P.S. contributed equally to this work.

The Imperial College London (under D.W.H.) and Centre d'Immunologie de Marseille-Luminy (under S.M.) groups participated equally in this work.

This article has been cited by other articles:

• Bujny, M. V., Ewels, P. A., Humphrey, S., Attar, N., Jepson, M. A., Cullen, P. J. (2008). Sorting nexin-1 defines an early phase of Salmonella-containing vacuole-remodeling during Salmonella infection. J. Cell Sci. 121: 2027-2036 [Abstract] [Full Text]  
• Wasylnka, J. A., Bakowski, M. A., Szeto, J., Ohlson, M. B., Trimble, W. S., Miller, S. I., Brumell, J. H. (2008). Role for Myosin II in Regulating Positioning of Salmonella-Containing Vacuoles and Intracellular Replication. Infect. Immun. 76: 2722-2735 [Abstract] [Full Text]