This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ono, T. Articles by Honda, T. Search for Related Content PubMed PubMed Citation Articles by Ono, T. Articles by Honda, T.

Identification of Proteins Secreted via Vibrio parahaemolyticus Type III Secretion System 1

Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan

Received 22 June 2005/ Returned for modification 12 August 2005/ Accepted 1 November 2005

Vibrio parahaemolyticus, a gram-negative marine bacterium, is an important pathogen causing food-borne gastroenteritis or septicemia. Recent genome sequencing of the RIMD2210633 strain (a Kanagawa phenomenon-positive clinical isolate of serotype O3:K6) revealed that the strain has two sets of gene clusters that encode the type III secretion system (TTSS) apparatus. The first cluster, TTSS1, is located on the large chromosome, and the second, TTSS2, is on the small chromosome. Previously, we reported that TTSS1 is involved in the cytotoxicity of the RIMD2210633 strain against HeLa cells. Here, we analyzed proteins secreted via the TTSS apparatus encoded by TTSS1 by using two-dimensional gel electrophoresis and identified the proteins encoded by genes VP1680, VP1686, and VPA450. To investigate the roles of those secreted proteins, we constructed and analyzed a series of deletion mutants. Flow cytometry analysis using fluorescence-activated cell sorting with fluorescein isothiocyanate-labeled annexin V demonstrated that the TTSS1-dependent cell death was by apoptosis. The cytotoxicity to HeLa cells was related to one of the newly identified secreted proteins encoded by VP1680. Adenylate cyclase fusion protein studies proved that the newly identified secreted proteins were translocated into HeLa cells. Thus, these appear to be the TTSS effector proteins in V. parahaemolyticus.

Editor: J. B. Bliska

This article has been cited by other articles:

• Casselli, T., Lynch, T., Southward, C. M., Jones, B. W., DeVinney, R. (2008). Vibrio parahaemolyticus Inhibition of Rho Family GTPase Activation Requires a Functional Chromosome I Type III Secretion System. Infect. Immun. 76: 2202-2211 [Abstract] [Full Text]  
• Coburn, B., Sekirov, I., Finlay, B. B. (2007). Type III Secretion Systems and Disease. Clin. Microbiol. Rev. 20: 535-549 [Abstract] [Full Text]  
• Meador, C. E., Parsons, M. M., Bopp, C. A., Gerner-Smidt, P., Painter, J. A., Vora, G. J. (2007). Virulence Gene- and Pandemic Group-Specific Marker Profiling of Clinical Vibrio parahaemolyticus Isolates. J. Clin. Microbiol. 45: 1133-1139 [Abstract] [Full Text]  
• Nair, G. B., Ramamurthy, T., Bhattacharya, S. K., Dutta, B., Takeda, Y., Sack, D. A. (2007). Global Dissemination of Vibrio parahaemolyticus Serotype O3:K6 and Its Serovariants. Clin. Microbiol. Rev. 20: 39-48 [Abstract] [Full Text]  
• Bhattacharjee, R. N., Park, K.-S., Kumagai, Y., Okada, K., Yamamoto, M., Uematsu, S., Matsui, K., Kumar, H., Kawai, T., Iida, T., Honda, T., Takeuchi, O., Akira, S. (2006). VP1686, a Vibrio Type III Secretion Protein, Induces Toll-like Receptor-independent Apoptosis in Macrophage through NF-{kappa}B Inhibition. J. Biol. Chem. 281: 36897-36904 [Abstract] [Full Text]  
• Thompson, F. L., Klose, K. E., the AVIB Group, (2006). Vibrio2005: the First International Conference on the Biology of Vibrios. J. Bacteriol. 188: 4592-4596 [Full Text]