This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bryan, A.
Right arrow Articles by Welch, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bryan, A.
Right arrow Articles by Welch, R. A.

 Previous Article  |  Next Article 

Infection and Immunity, February 2006, p. 1072-1083, Vol. 74, No. 2
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.2.1072-1083.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Regulation of Type 1 Fimbriae by Unlinked FimB- and FimE-Like Recombinases in Uropathogenic Escherichia coli Strain CFT073

Andrew Bryan, Paula Roesch, Lindsay Davis, Rebecca Moritz, Shahaireen Pellett, and Rodney A. Welch*

Department of Medical Microbiology and Immunology, University of Wisconsin—Madison, Madison, Wisconsin 53706

Received 23 September 2005/ Returned for modification 3 November 2005/ Accepted 17 November 2005

Genomic DNA sequence analysis of the uropathogenic Escherichia coli strain CFT073 revealed that besides the fimB and fimE recombinase genes that control the type 1 pilus fim phase switch, there are three additional fimB- and fimE-like genes: ipuA, ipuB, and ipbA. Alignment of the predicted amino acid sequences showed that the five recombinases range in sequence similarity from 63 to 70%. An epidemiological survey indicates that ipuA and ipuB are present and linked next to the dsdCXA locus in 24 of 67 uropathogenic E. coli strains but are found in only 1 of 15 normal human fecal isolates. The ipbA sequence located next to the betABIT locus was found in 42 of 67 uropathogenic isolates and 8 of 15 of the commensal strains. We show that two of these recombinases, those encoded by ipuA and ipbA, can function at the type 1 pilus fim switch. In a CFT073 deletion mutant lacking all five recombinase genes, recombinant ipuA or ipbA provided in trans inverted the fim element from the off state to the on state. When a fim OFF CFT073 {Delta}fimBE mutant was used to infect the urinary tracts of mice, a switch to the fim on state was detected within 24 h in bacteria recovered from urine, the bladder, and the kidneys. A fim OFF CFT073 {Delta}fimBE ipuB ipbA mutant also demonstrated the ability to switch from the fim off state to the on state during mouse infection. CFT073 recombinase mutants derived from isolates in either the fim on or off state showed a reciprocal relationship for motility. Switches from a nonmotile to a motile phenotype and from a fim on to off genotype were observed in fim ON CFT073 {Delta}fimBE ipuAB ipbA mutants when ipuA or fimB was provided in trans. Together these results indicate that ipuA has fimB-like on-to-off and off-to-on fim switching activity and that ipbA has the ability to switch fim from the off to the on orientation.

* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, University of Wisconsin—Madison, Madison, WI 53706. Phone: (608) 263-2700. Fax: (608) 262-8418. E-mail: rawelch{at}wisc.edu.

Editor: A. D. O'Brien

Infection and Immunity, February 2006, p. 1072-1083, Vol. 74, No. 2
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.2.1072-1083.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Bower, J. M., Gordon-Raagas, H. B., Mulvey, M. A. (2009). Conditioning of Uropathogenic Escherichia coli for Enhanced Colonization of Host. Infect. Immun. 77: 2104-2112 [Abstract] [Full Text]  
  • Lane, M. C., Li, X., Pearson, M. M., Simms, A. N., Mobley, H. L. T. (2009). Oxygen-Limiting Conditions Enrich for Fimbriate Cells of Uropathogenic Proteus mirabilis and Escherichia coli. J. Bacteriol. 191: 1382-1392 [Abstract] [Full Text]  
  • Cortes, M. A. M., Gibon, J., Chanteloup, N. K., Moulin-Schouleur, M., Gilot, P., Germon, P. (2008). Inactivation of ibeA and ibeT Results in Decreased Expression of Type 1 Fimbriae in Extraintestinal Pathogenic Escherichia coli Strain BEN2908. Infect. Immun. 76: 4129-4136 [Abstract] [Full Text]  
  • Rosen, D. A., Pinkner, J. S., Jones, J. M., Walker, J. N., Clegg, S., Hultgren, S. J. (2008). Utilization of an Intracellular Bacterial Community Pathway in Klebsiella pneumoniae Urinary Tract Infection and the Effects of FimK on Type 1 Pilus Expression. Infect. Immun. 76: 3337-3345 [Abstract] [Full Text]  
  • Rosen, D. A., Pinkner, J. S., Walker, J. N., Elam, J. S., Jones, J. M., Hultgren, S. J. (2008). Molecular Variations in Klebsiella pneumoniae and Escherichia coli FimH Affect Function and Pathogenesis in the Urinary Tract. Infect. Immun. 76: 3346-3356 [Abstract] [Full Text]  
  • Simms, A. N., Mobley, H. L. T. (2008). Multiple Genes Repress Motility in Uropathogenic Escherichia coli Constitutively Expressing Type 1 Fimbriae. J. Bacteriol. 190: 3747-3756 [Abstract] [Full Text]  
  • Anfora, A. T., Haugen, B. J., Roesch, P., Redford, P., Welch, R. A. (2007). Roles of Serine Accumulation and Catabolism in the Colonization of the Murine Urinary Tract by Escherichia coli CFT073. Infect. Immun. 75: 5298-5304 [Abstract] [Full Text]  
  • Lane, M. C., Simms, A. N., Mobley, H. L. T. (2007). Complex Interplay between Type 1 Fimbrial Expression and Flagellum-Mediated Motility of Uropathogenic Escherichia coli. J. Bacteriol. 189: 5523-5533 [Abstract] [Full Text]  
  • Ulett, G. C., Valle, J., Beloin, C., Sherlock, O., Ghigo, J.-M., Schembri, M. A. (2007). Functional Analysis of Antigen 43 in Uropathogenic Escherichia coli Reveals a Role in Long-Term Persistence in the Urinary Tract. Infect. Immun. 75: 3233-3244 [Abstract] [Full Text]  
  • Haugen, B. J., Pellett, S., Redford, P., Hamilton, H. L., Roesch, P. L., Welch, R. A. (2007). In Vivo Gene Expression Analysis Identifies Genes Required for Enhanced Colonization of the Mouse Urinary Tract by Uropathogenic Escherichia coli Strain CFT073 dsdA. Infect. Immun. 75: 278-289 [Abstract] [Full Text]  
  • Moritz, R. L., Welch, R. A. (2006). The Escherichia coli argW-dsdCXA Genetic Island Is Highly Variable, and E. coli K1 Strains Commonly Possess Two Copies of dsdCXA.. J. Clin. Microbiol. 44: 4038-4048 [Abstract] [Full Text]  
  • Xie, Y., Yao, Y., Kolisnychenko, V., Teng, C.-H., Kim, K. S. (2006). HbiF Regulates Type 1 Fimbriation Independently of FimB and FimE. Infect. Immun. 74: 4039-4047 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»