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Infection and Immunity, February 2006, p. 1084-1090, Vol. 74, No. 2
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.2.1084-1090.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Cre Reporter System To Monitor the Translocation of Type III Secreted Proteins into Host Cells

Gabriel Briones, Dirk Hofreuter, and Jorge E. Galán*

Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, Connecticut 06536

Received 31 August 2005/ Returned for modification 21 October 2005/ Accepted 27 November 2005

Central to the study of type III secretion systems is the availability of reporter systems to monitor bacterial protein translocation into host cells. We report here the development of a bacteriophage P1 Cre recombinase-based system to monitor the translocation of bacterial proteins into mammalian cells. Bacteriophage P1 Cre recombinase fused to the secretion and translocation signals of Salmonella enterica serovar Typhimurium of the type III secreted protein SopE was secreted in a type III secretion system-dependent fashion. More importantly, the SopE-Cre chimera was translocated into host cells via the type III secretion system and activated the expression of luciferase and green fluorescent protein reporters of Cre recombinase activity.

* Corresponding author. Mailing address: Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06536. Phone: (203) 737-2404. Fax: (203) 737-2630. E-mail: jorge.galan{at}yale.edu.

Editor: J. B. Bliska

Infection and Immunity, February 2006, p. 1084-1090, Vol. 74, No. 2
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.2.1084-1090.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Torruellas Garcia, J., Ferracci, F., Jackson, M. W., Joseph, S. S., Pattis, I., Plano, L. R. W., Fischer, W., Plano, G. V. (2006). Measurement of Effector Protein Injection by Type III and Type IV Secretion Systems by Using a 13-Residue Phosphorylatable Glycogen Synthase Kinase Tag.. Infect. Immun. 74: 5645-5657 [Abstract] [Full Text]  


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