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Infection and Immunity, February 2006, p. 1171-1180, Vol. 74, No. 2
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.2.1171-1180.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Mn2+-Dependent Regulation of Multiple Genes in Streptococcus pneumoniae through PsaR and the Resultant Impact on Virulence

Jason W. Johnston,1* David E. Briles,1 Lisa E. Myers,2 and Susan K. Hollingshead1

Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294,1 Department of Microbiology, Sanofi Pasteur, 1755 Steeles Avenue West, Toronto, Ontario, Canada M2R 3T42

Received 12 July 2005/ Returned for modification 26 August 2005/ Accepted 16 November 2005

The concentration of Mn2+ is 1,000-fold higher in secretions than it is at internal sites of the body, making it a potential signal by which bacteria can sense a shift from a mucosal environment to a more invasive site. PsaR, a metal-dependent regulator in Streptococcus pneumoniae, was found to negatively affect the transcription of psaBCA, pcpA, rrgA, rrgB, rrgC, srtBCD, and rlrA in the presence of Mn2+. psaBCA encode an ABC-type transporter for Mn2+. pcpA, rrgA, rrgB, and rrgC encode several outer surface proteins. srtBCD encode a cluster of sortase enzymes, and rlrA encodes a transcriptional regulator. Steady-state RNA levels are high under low Mn2+ concentrations in the wild-type strain and are elevated under both high and low Mn2+ concentrations in a psaR mutant strain. RlrA is an activator of rrgA, rrgB, rrgC, and srtBCD (D. Hava and A. Camilli, Mol. Microbiol. 45:1389-1406, 2002), suggesting that PsaR may indirectly control these genes through rlrA, while PsaR-dependent repression of psaBCA, pcpA, and rlrA transcription is direct. The impact of Mn2+-dependent regulation on virulence was further examined in mouse models of pneumonia and nasopharyngeal carriage. The abilities of {Delta}psaR, pcpA, and {Delta}psaR {Delta}pcpA mutant strains to colonize the lung were reduced compared to those of the wild type, confirming that both PcpA-mediated gene regulation and PsaR-mediated gene regulation are required for full virulence in the establishment of pneumonia. Neither PcpA nor PsaR was found to be required for colonization of the nasopharynx in a carriage model. This is the first demonstration of Mn2+ acting as a signal for the expression of virulence factors within different host sites.

* Corresponding author. Present address: Department of Microbiology, University of Iowa, BSB 3-401, 51 Newton Road, Iowa City, IA 52242. Phone: (319) 353-5654. Fax: (319) 335-9006. E-mail: jason-johnston{at}uiowa. edu.

Editor: J. N. Weiser

Infection and Immunity, February 2006, p. 1171-1180, Vol. 74, No. 2
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.2.1171-1180.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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