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Infection and Immunity, February 2006, p. 1381-1386, Vol. 74, No. 2
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.2.1381-1386.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Calcium-Regulated Type III Secretion of Yop Proteins by an Escherichia coli hha Mutant Carrying a Yersinia pestis pCD1 Virulence Plasmid

Sara Schesser Bartra, Michael W. Jackson, Julia A. Ross, and Gregory V. Plano*

Department of Microbiology and Immunology, University of Miami Miller School of Medicine, P.O. Box 016960 (R-138), Miami, Florida 33101

Received 8 August 2005/ Returned for modification 14 September 2005/ Accepted 19 October 2005

A series of four large deletions that removed a total of ca. 36 kb of DNA from the ca. 70-kb Yersinia pestis pCD1 virulence plasmid were constructed using lambda Red-mediated recombination. Escherichia coli hha deletion mutants carrying the virulence plasmid with the deletions expressed a functional calcium-regulated type III secretion system. The E. coli hha/pCD1 system should facilitate molecular studies of the type III secretion process.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Miami Miller School of Medicine, P.O. Box 016960 (R-138), Miami, FL 33101. Phone: (305) 243-6310. Fax: (305) 243-4623. E-mail: gplano{at}med.miami.edu.

Editor: J. B. Bliska

Infection and Immunity, February 2006, p. 1381-1386, Vol. 74, No. 2
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.2.1381-1386.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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