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Infection and Immunity, February 2006, p. 869-875, Vol. 74, No. 2
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.2.869-875.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Importance of Heat-Labile Enterotoxin in Colonization of the Adult Mouse Small Intestine by Human Enterotoxigenic Escherichia coli Strains

Kenneth P. Allen,1 Mildred M. Randolph,1 and James M. Fleckenstein2,3,4*

Departments of Comparative Medicine,1 Medicine,2 Molecular Sciences, University of Tennessee Health Science Center, Memphis, Tennessee,3 Medicine Service, Veterans Affairs Medical Center, Memphis, Tennessee4

Received 28 April 2005/ Returned for modification 1 August 2005/ Accepted 21 October 2005

Enterotoxigenic Escherichia coli (ETEC) infections are a significant cause of diarrheal disease and infant mortality in developing countries. Studies of ETEC pathogenesis relevant to vaccine development have been greatly hampered by the lack of a suitable small-animal model of infection with human ETEC strains. Here, we demonstrate that adult immunocompetent outbred mice can be effectively colonized with the prototypical human ETEC H10407 strain (colonization factor antigen I; heat-labile and heat-stable enterotoxin positive) and that production of heat-labile holotoxin provides a significant advantage in colonization of the small intestine in this model.

* Corresponding author. Mailing address: Research Service (151), Veterans Affairs Medical Center, 1030 Jefferson Avenue, Memphis, TN 38104. Phone: (901) 523-8990, ext. 7547. Fax: (901) 577-7273. E-mail: jflecke1{at}tennessee.edu.

Editor: V. J. DiRita

Infection and Immunity, February 2006, p. 869-875, Vol. 74, No. 2
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.2.869-875.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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