This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sharma, J.
Right arrow Articles by Zhong, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sharma, J.
Right arrow Articles by Zhong, G.

 Previous Article  |  Next Article 

Infection and Immunity, March 2006, p. 1490-1499, Vol. 74, No. 3
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.3.1490-1499.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Profiling of Human Antibody Responses to Chlamydia trachomatis Urogenital Tract Infection Using Microplates Arrayed with 156 Chlamydial Fusion Proteins

Jyotika Sharma,1 Youmin Zhong,1 Feng Dong,1 Jeanna M. Piper,2 Guqi Wang,1 and Guangming Zhong1*

Departments of Microbiology and Immunology,1 Obstetrics and Genecology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, Texas 782292

Received 18 June 2005/ Returned for modification 4 July 2005/ Accepted 22 November 2005

The available chlamydial genome sequences have made it possible to comprehensively analyze host responses to all chlamydial proteins, which is essential for further understanding of chlamydial pathogenesis and development of effective chlamydial vaccines. Microplates arrayed with 156 Chlamydia trachomatis fusion proteins were used to evaluate antibody responses in women urogenitally infected with C. trachomatis. Based on both the antibody recognition frequency and titer, seven chlamydial antigens encoded by open reading frames (ORFs) CT089, CT147, CT226, CT681, CT694, CT795, and CT858, respectively, were identified as relatively immunodominant; six of these are encoded by hypothetical ORFs. Antibody binding to these chlamydial fusion proteins was blocked by C. trachomatis-infected but not by normal HeLa cell lysates or irrelevant bacterial lysates. These results have revealed novel immune-reactive chlamydial antigens, not only indicating that the hypothetical ORF-encoded proteins are expressed during chlamydial infection in humans but also providing the proof of principle that the fusion protein-based approach can be used to profile human immune responses to chlamydial infection at the whole-genome scale.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229. Phone: (210) 567-1169. Fax: (210) 567-0293. E-mail: Zhongg{at}UTHSCSA.EDU.

Editor: F. C. Fang

Infection and Immunity, March 2006, p. 1490-1499, Vol. 74, No. 3
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.3.1490-1499.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Wills, G. S., Horner, P. J., Reynolds, R., Johnson, A. M., Muir, D. A., Brown, D. W., Winston, A., Broadbent, A. J., Parker, D., McClure, M. O. (2009). Pgp3 Antibody Enzyme-Linked Immunosorbent Assay, a Sensitive and Specific Assay for Seroepidemiological Analysis of Chlamydia trachomatis Infection. CVI 16: 835-843 [Abstract] [Full Text]  
  • Beare, P. A., Chen, C., Bouman, T., Pablo, J., Unal, B., Cockrell, D. C., Brown, W. C., Barbian, K. D., Porcella, S. F., Samuel, J. E., Felgner, P. L., Heinzen, R. A. (2008). Candidate Antigens for Q Fever Serodiagnosis Revealed by Immunoscreening of a Coxiella burnetii Protein Microarray. CVI 15: 1771-1779 [Abstract] [Full Text]  
  • Li, Z., Chen, D., Zhong, Y., Wang, S., Zhong, G. (2008). The Chlamydial Plasmid-Encoded Protein pgp3 Is Secreted into the Cytosol of Chlamydia-Infected Cells. Infect. Immun. 76: 3415-3428 [Abstract] [Full Text]  
  • Li, Z., Chen, C., Chen, D., Wu, Y., Zhong, Y., Zhong, G. (2008). Characterization of Fifty Putative Inclusion Membrane Proteins Encoded in the Chlamydia trachomatis Genome. Infect. Immun. 76: 2746-2757 [Abstract] [Full Text]  
  • Bunk, S., Susnea, I., Rupp, J., Summersgill, J. T., Maass, M., Stegmann, W., Schrattenholz, A., Wendel, A., Przybylski, M., Hermann, C. (2008). Immunoproteomic Identification and Serological Responses to Novel Chlamydia pneumoniae Antigens That Are Associated with Persistent C. pneumoniae Infections. J. Immunol. 180: 5490-5498 [Abstract] [Full Text]  
  • DeMars, R., Weinfurter, J. (2008). Interstrain Gene Transfer in Chlamydia trachomatis In Vitro: Mechanism and Significance. J. Bacteriol. 190: 1605-1614 [Abstract] [Full Text]  
  • Li, W., Murthy, A. K., Guentzel, M. N., Seshu, J., Forsthuber, T. G., Zhong, G., Arulanandam, B. P. (2008). Antigen-Specific CD4+ T Cells Produce Sufficient IFN-{gamma} to Mediate Robust Protective Immunity against Genital Chlamydia muridarum Infection. J. Immunol. 180: 3375-3382 [Abstract] [Full Text]  
  • Li, W., Guentzel, M. N., Seshu, J., Zhong, G., Murthy, A. K., Arulanandam, B. P. (2007). Induction of Cross-Serovar Protection against Genital Chlamydial Infection by a Targeted Multisubunit Vaccination Approach. CVI 14: 1537-1544 [Abstract] [Full Text]  
  • Parekh, F. K., Richie, T. L. (2007). Characterization of Immune Reactivity Profiles Using Microarray Technology May Expedite Identification of Candidate Antigens for Next Generation Malaria Vaccines. Clin. Chem. 53: 1183-1185 [Full Text]  
  • Flores, R., Luo, J., Chen, D., Sturgeon, G., Shivshankar, P., Zhong, Y., Zhong, G. (2007). Characterization of the hypothetical protein Cpn1027, a newly identified inclusion membrane protein unique to Chlamydia pneumoniae. Microbiology 153: 777-786 [Abstract] [Full Text]  
  • Murthy, A. K., Chambers, J. P., Meier, P. A., Zhong, G., Arulanandam, B. P. (2007). Intranasal Vaccination with a Secreted Chlamydial Protein Enhances Resolution of Genital Chlamydia muridarum Infection, Protects against Oviduct Pathology, and Is Highly Dependent upon Endogenous Gamma Interferon Production. Infect. Immun. 75: 666-676 [Abstract] [Full Text]  
  • Luo, J., Jia, T., Flores, R., Chen, D., Zhong, G. (2007). Hypothetical Protein Cpn0308 Is Localized in the Chlamydia pneumoniae Inclusion Membrane. Infect. Immun. 75: 497-503 [Abstract] [Full Text]  
  • Dong, F., Flores, R., Chen, D., Luo, J., Zhong, Y., Wu, Z., Zhong, G. (2006). Localization of the Hypothetical Protein Cpn0797 in the Cytoplasm of Chlamydia pneumoniae-Infected Host Cells.. Infect. Immun. 74: 6479-6486 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»