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Infection and Immunity, March 2006, p. 1500-1504, Vol. 74, No. 3
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.3.1500-1504.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Estimating the Relative Contributions of Virulence Factors for Pathogenic Microbes{dagger}

Erin E. McClelland,1* Paul Bernhardt,2 and Arturo Casadevall1

Department of Medicine, 702 Golding, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461,1 Department of Psychology, Westminster College, 1840 South 1300 East, Salt Lake City, Utah 841052

Received 6 November 2005/ Returned for modification 23 November 2005/ Accepted 18 December 2005

Many pathogenic microbes have multiple virulence factors that can cause damage to the host and thus contribute to an overall virulence phenotype for that organism. Although current techniques are suitable for demonstrating that a particular microbial characteristic contributes to virulence, no formal approach for defining the relative contributions of multiple virulence factors to overall virulence has been proposed. This paper describes the use of multivariate linear regression to estimate the relative contributions of virulence factors to the overall phenomenon of virulence. The approach is illustrated here with sample calculations of the relative contributions of individual Cryptococcus neoformans and Bacillus anthracis virulence factors to the overall virulence phenotype. These calculations were derived from a small underpowered experimental data set for the fungus and two larger sets of randomly generated data for both microbes. The major limitation of this method is a requirement for large data sets of microbial strains that differ in virulence and virulence factor expression. Multivariate linear regression can be used to identify the relative levels of importance of virulence factors in virulence studies, and this information can be used to prioritize antigen identification for vaccine development and the design of antimicrobial strategies that target virulence mechanisms.

* Corresponding author. Mailing address: Department of Medicine, 702 Golding, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461. Phone: (718) 430-3768. Fax: (718) 430-8701. E-mail: mcclella{at}aecom.yu.edu.

{dagger} Supplemental material for this article may be found at http://iai.asm.org/.

Editor: W. A. Petri, Jr.

Infection and Immunity, March 2006, p. 1500-1504, Vol. 74, No. 3
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.3.1500-1504.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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