This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thomas, C. E.
Right arrow Articles by Sparling, P. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thomas, C. E.
Right arrow Articles by Sparling, P. F.

 Previous Article  |  Next Article 

Infection and Immunity, March 2006, p. 1612-1620, Vol. 74, No. 3
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.3.1612-1620.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Vaccination of Mice with Gonococcal TbpB Expressed In Vivo from Venezuelan Equine Encephalitis Viral Replicon Particles

Christopher E. Thomas,1* Weiyan Zhu,1 Cornelius N. Van Dam,1 Nancy L. Davis,2 Robert E. Johnston,2 and P. Frederick Sparling1,2

Department of Medicine, Division of Infectious Diseases,1 Department of Microbiology and Immunology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina2

Received 10 August 2005/ Returned for modification 1 November 2005/ Accepted 22 December 2005

We investigated the immunogenicity of gonococcal transferrin binding protein B (TbpB) expressed with and without a eukaryotic secretion signal from a nonpropagating Venezuelan equine encephalitis virus replicon particle (VRP) delivery system. TbpB was successfully expressed in baby hamster kidney (BHK) cells, and the presence of the eukaryotic secretion signal not only apparently increased the protein's expression but also allowed for extracellular localization and glycosylation. Mice immunized with VRPs produced significant amounts of serum antibody although less than the amounts produced by mice immunized with recombinant protein. The response of mice immunized with VRPs encoding TbpB was consistently more Th1 biased than the response of mice immunized with recombinant protein alone. Boosting with recombinant protein following immunization with TbpB VRPs resulted in higher specific-antibody levels without altering the Th1/Th2 bias. Most of the immunization groups produced significant specific antibody binding to the intact surface of the homologous Neisseria gonorrhoeae strain. Immunization with TbpB VRPs without a eukaryotic secretion signal generated no measurable specific antibodies on the genital mucosal surface, but inclusion of a eukaryotic secretion signal or boosting with recombinant protein resulted in specific immunoglobulin G (IgG) and IgA in mucosal secretions after TbpB VRP immunization. The TbpB VRP system has potential for an N. gonorrhoeae vaccine.

* Corresponding author. Mailing address: University of North Carolina at Chapel Hill, Dept. of Medicine, Div. of Infectious Disease Research, 8341 Medical Biomolecular Research Bldg., 103 Mason Farm Road, CB 7031, Chapel Hill, NC 27599. Phone: (919) 966-3661. Fax: (919) 843-1015. E-mail: cet{at}med.unc.edu.

Editor: D. L. Burns

Infection and Immunity, March 2006, p. 1612-1620, Vol. 74, No. 3
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.3.1612-1620.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mok, H., Tollefson, S. J., Podsiad, A. B., Shepherd, B. E., Polosukhin, V. V., Johnston, R. E., Williams, J. V., Crowe, J. E. Jr (2008). An Alphavirus Replicon-Based Human Metapneumovirus Vaccine Is Immunogenic and Protective in Mice and Cotton Rats. J. Virol. 82: 11410-11418 [Abstract] [Full Text]  
  • Noto, J. M., Cornelissen, C. N. (2008). Identification of TbpA Residues Required for Transferrin-Iron Utilization by Neisseria gonorrhoeae. Infect. Immun. 76: 1960-1969 [Abstract] [Full Text]  
  • DeRocco, A. J., Cornelissen, C. N. (2007). Identification of Transferrin-Binding Domains in TbpB Expressed by Neisseria gonorrhoeae. Infect. Immun. 75: 3220-3232 [Abstract] [Full Text]  
  • Durso, R. J., Andjelic, S., Gardner, J. P., Margitich, D. J., Donovan, G. P., Arrigale, R. R., Wang, X., Maughan, M. F., Talarico, T. L., Olmsted, R. A., Heston, W. D.W., Maddon, P. J., Olson, W. C. (2007). A Novel Alphavirus Vaccine Encoding Prostate-Specific Membrane Antigen Elicits Potent Cellular and Humoral Immune Responses. Clin. Cancer Res. 13: 3999-4008 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»