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Infection and Immunity, March 2006, p. 1621-1630, Vol. 74, No. 3
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.3.1621-1630.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Role of Phagosomes and Major Histocompatibility Complex Class II (MHC-II) Compartment in MHC-II Antigen Processing of Mycobacterium tuberculosis in Human Macrophages

Martha Torres,1,5,{dagger} Lakshmi Ramachandra,2,4,{dagger}* Roxana E. Rojas,1 Karen Bobadilla,5 Jeremy Thomas,1 David H. Canaday,1 Clifford V. Harding,3,{ddagger} and W. Henry Boom1,{ddagger}

Division of Infectious Disease,1 Department of Pediatrics,2 Department of Pathology, Case Western Reserve University, Cleveland, Ohio,3 Rainbow Babies and Children's Hospital, Cleveland, Ohio,4 Departamento de Microbiologia, Instituto Nacional de Enfermedades Respiratorias, Mexico City, Mexico5

Received 7 September 2005/ Returned for modification 13 October 2005/ Accepted 7 December 2005

Mycobacterium tuberculosis resides in phagosomes inside macrophages. In this study, we analyzed the kinetics and location of M. tuberculosis peptide-major histocompatibility complex class II (MHC-II) complexes in M. tuberculosis-infected human macrophages. M. tuberculosis peptide-MHC-II complexes were detected with polyclonal autologous M. tuberculosis-specific CD4+ T cells or F9A6 T hybridoma cells specific for M. tuberculosis antigen (Ag) 85B (96-111). Macrophages processed heat-killed M. tuberculosis more rapidly and efficiently than live M. tuberculosis. To determine where M. tuberculosis peptide-MHC-II complexes were formed intracellularly, macrophages incubated with heat-killed M. tuberculosis were homogenized, and subcellular compartments were separated on Percoll density gradients analyzed with T cells. In THP-1 cells, M. tuberculosis Ag 85B (96- 111)-DR1 complexes appeared initially in phagosomes, followed by MHC class II compartment (MIIC) and the plasma membrane fractions. In monocyte-derived macrophages, M. tuberculosis peptide-MHC-II complexes appeared only in MIIC fractions and subsequently on the plasma membrane. Although phagosomes from both cell types acquired lysosome-associated membrane protein 1 (LAMP-1) and MHC-II, THP-1 phagosomes that support formation of M. tuberculosis peptide-MHC-II complexes had increased levels of both LAMP-1 and MHC-II. Thus, M. tuberculosis phagosomes with high levels of MHC-II and LAMP-1 and MIIC both have the potential to form peptide-MHC-II complexes from M. tuberculosis antigens in human macrophages.

* Corresponding author. Mailing address: Division of Infectious Diseases, Dept. of Pediatrics, Rainbow Babies and Children's Hospital, Rm. 4007, 2101 Adelbert Rd., Cleveland, OH 44106-6008. Phone: (216) 844-5873. Fax: (216) 844-8362. E-mail: lxr2{at}cwru.edu.

Editor: W. A. Petri, Jr.

{dagger} These two authors (Martha Torres and Lakshmi Ramachandra) contributed equally to this work.

{ddagger} These two authors share senior authorship.

Infection and Immunity, March 2006, p. 1621-1630, Vol. 74, No. 3
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.3.1621-1630.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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