This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Palaniappan, R. U. M.
Right arrow Articles by Chang, Y.-F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Palaniappan, R. U. M.
Right arrow Articles by Chang, Y.-F.

 Previous Article  |  Next Article 

Infection and Immunity, March 2006, p. 1745-1750, Vol. 74, No. 3
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.3.1745-1750.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Immunoprotection of Recombinant Leptospiral Immunoglobulin-Like Protein A against Leptospira interrogans Serovar Pomona Infection

Raghavan U. M. Palaniappan,1 Sean P. McDonough,1 Thomas J. Divers,1 Chia-Sui Chen,1 Ming-Jeng Pan,2 Mitsuharu Matsumoto,1 and Yung-Fu Chang1*

College of Veterinary Medicine, Cornell University, Ithaca, New York 14853,1 Institute of Medical Biotechnology, Central Taiwan University of Science and Technology, Taichung, Taiwan2

Received 15 November 2005/ Accepted 2 December 2005

We previously reported the cloning and characterization of leptospiral immunoglobulin-like proteins LigA and LigB of Leptospira interrogans. LigA and LigB are conserved at the amino-terminal region but are variable at the carboxyl-terminal region. Here, we evaluate the potential of recombinant LigA (rLigA) as a vaccine candidate against infection by L. interrogans serovar Pomona in a hamster model. rLigA was truncated into conserved (rLigAcon) and variable (rLigAvar) regions and expressed in Escherichia coli as a fusion protein with glutathione-S-transferase (rLigA). Golden Syrian hamsters were immunized at 3 and 6 weeks of age with rLigA (rLigAcon and rLigAvar) with aluminum hydroxide as an adjuvant. Hamsters given recombinant glutathione-S-transferase (rGST)-adjuvant and phosphate-buffered saline-adjuvant served as nonvaccinated controls. Three weeks after the last vaccination, all animals were challenged intraperitoneally with 108 L. interrogans serovar Pomona bacteria (NVSL 1427-35-093002). All hamsters immunized with recombinant LigA survived after challenge and had no significant histopathological changes. In contrast, nonimmunized and rGST-immunized hamsters were subjected to lethal doses, and the hamsters that survived showed severe tubulointerstitial nephritis. All vaccinated animals showed a rise in antibody titers against rLigA. Results from this study indicate that rLigA is a potential vaccine candidate against L. interrogans serovar Pomona infection.

* Corresponding author. Mailing address: Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853. Phone: (607) 253-3675. Fax: (607) 253-3943. E-mail: yc42{at}cornell.edu.

Editor: D. L. Burns

Infection and Immunity, March 2006, p. 1745-1750, Vol. 74, No. 3
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.3.1745-1750.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Croda, J., Figueira, C. P., Wunder, E. A. Jr., Santos, C. S., Reis, M. G., Ko, A. I., Picardeau, M. (2008). Targeted Mutagenesis in Pathogenic Leptospira Species: Disruption of the LigB Gene Does Not Affect Virulence in Animal Models of Leptospirosis. Infect. Immun. 76: 5826-5833 [Abstract] [Full Text]  
  • Lin, Y.-P., Raman, R., Sharma, Y., Chang, Y.-F. (2008). Calcium Binds to Leptospiral Immunoglobulin-like Protein, LigB, and Modulates Fibronectin Binding. J. Biol. Chem. 283: 25140-25149 [Abstract] [Full Text]  
  • Choy, H. A., Kelley, M. M., Chen, T. L., Moller, A. K., Matsunaga, J., Haake, D. A. (2007). Physiological Osmotic Induction of Leptospira interrogans Adhesion: LigA and LigB Bind Extracellular Matrix Proteins and Fibrinogen. Infect. Immun. 75: 2441-2450 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»