This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Janes, B. K.
Right arrow Articles by Stibitz, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Janes, B. K.
Right arrow Articles by Stibitz, S.

 Previous Article  |  Next Article 

Infection and Immunity, March 2006, p. 1949-1953, Vol. 74, No. 3
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.3.1949-1953.2006

Routine Markerless Gene Replacement in Bacillus anthracis

Brian K. Janes{dagger} and Scott Stibitz*

Division of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, 8800 Rockville Pike, Bethesda, Maryland 20892

Received 2 November 2005/ Returned for modification 22 November 2005/ Accepted 8 December 2005

An improved genetic tool suitable for routine markerless allelic exchange in Bacillus anthracis has been constructed. Its utility was demonstrated by the introduction of insertions, deletions, and missense mutations on the chromosome and plasmid pXO1 of the Sterne strain of B. anthracis.

* Corresponding author. Mailing address: Division of Bacterial Products, CBER/FDA, 8800 Rockville Pike, Bethesda, MD 20892. Phone: (301) 827-5156. Fax: (301) 402-2776. E-mail: stibitz{at}helix.nih.gov.

Editor: J. T. Barbieri

{dagger} Present address: Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Mich.

Infection and Immunity, March 2006, p. 1949-1953, Vol. 74, No. 3
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.3.1949-1953.2006




This article has been cited by other articles:

  • Ebrahimi, C. M., Kern, J. W., Sheen, T. R., Ebrahimi-Fardooee, M. A., van Sorge, N. M., Schneewind, O., Doran, K. S. (2009). Penetration of the Blood-Brain Barrier by Bacillus anthracis Requires the pXO1-Encoded BslA Protein. J. Bacteriol. 191: 7165-7173 [Abstract] [Full Text]  
  • Lopez, C. M., Rholl, D. A., Trunck, L. A., Schweizer, H. P. (2009). Versatile Dual-Technology System for Markerless Allele Replacement in Burkholderia pseudomallei. Appl. Environ. Microbiol. 75: 6496-6503 [Abstract] [Full Text]  
  • Vasudevan, P., McElligott, J., Attkisson, C., Betteken, M., Popham, D. L. (2009). Homologues of the Bacillus subtilis SpoVB Protein Are Involved in Cell Wall Metabolism. J. Bacteriol. 191: 6012-6019 [Abstract] [Full Text]  
  • Giebel, J. D., Carr, K. A., Anderson, E. C., Hanna, P. C. (2009). The Germination-Specific Lytic Enzymes SleB, CwlJ1, and CwlJ2 Each Contribute to Bacillus anthracis Spore Germination and Virulence. J. Bacteriol. 191: 5569-5576 [Abstract] [Full Text]  
  • Pomerantsev, A. P., Camp, A., Leppla, S. H. (2009). A New Minimal Replicon of Bacillus anthracis Plasmid pXO1. J. Bacteriol. 191: 5134-5146 [Abstract] [Full Text]  
  • Chandramohan, L., Ahn, J.-S., Weaver, K. E., Bayles, K. W. (2009). An Overlap between the Control of Programmed Cell Death in Bacillus anthracis and Sporulation. J. Bacteriol. 191: 4103-4110 [Abstract] [Full Text]  
  • Heffron, J. D., Orsburn, B., Popham, D. L. (2009). Roles of Germination-Specific Lytic Enzymes CwlJ and SleB in Bacillus anthracis. J. Bacteriol. 191: 2237-2247 [Abstract] [Full Text]  
  • Cybulski, R. J. Jr., Sanz, P., Alem, F., Stibitz, S., Bull, R. L., O'Brien, A. D. (2009). Four Superoxide Dismutases Contribute to Bacillus anthracis Virulence and Provide Spores with Redundant Protection from Oxidative Stress. Infect. Immun. 77: 274-285 [Abstract] [Full Text]  
  • Loving, C. L., Khurana, T., Osorio, M., Lee, G. M., Kelly, V. K., Stibitz, S., Merkel, T. J. (2009). Role of Anthrax Toxins in Dissemination, Disease Progression, and Induction of Protective Adaptive Immunity in the Mouse Aerosol Challenge Model. Infect. Immun. 77: 255-265 [Abstract] [Full Text]  
  • Lambert, E. A., Popham, D. L. (2008). The Bacillus anthracis SleL (YaaH) Protein Is an N-Acetylglucosaminidase Involved in Spore Cortex Depolymerization. J. Bacteriol. 190: 7601-7607 [Abstract] [Full Text]  
  • Bongiorni, C., Fukushima, T., Wilson, A. C., Chiang, C., Mansilla, M. C., Hoch, J. A., Perego, M. (2008). Dual Promoters Control Expression of the Bacillus anthracis Virulence Factor AtxA. J. Bacteriol. 190: 6483-6492 [Abstract] [Full Text]  
  • Paige, C., Reid, S. D., Hanna, P. C., Claiborne, A. (2008). The Type III Pantothenate Kinase Encoded by coaX Is Essential for Growth of Bacillus anthracis. J. Bacteriol. 190: 6271-6275 [Abstract] [Full Text]  
  • Wilson, A. C., Hoch, J. A., Perego, M. (2008). Virulence Gene Expression Is Independent of ResDE-Regulated Respiration Control in Bacillus anthracis. J. Bacteriol. 190: 5522-5525 [Abstract] [Full Text]  
  • Hsu, L.-C., Ali, S. R., McGillivray, S., Tseng, P.-H., Mariathasan, S., Humke, E. W., Eckmann, L., Powell, J. J., Nizet, V., Dixit, V. M., Karin, M. (2008). A NOD2-NALP1 complex mediates caspase-1-dependent IL-1{beta} secretion in response to Bacillus anthracis infection and muramyl dipeptide. Proc. Natl. Acad. Sci. USA 105: 7803-7808 [Abstract] [Full Text]  
  • Brahmbhatt, T. N., Janes, B. K., Stibitz, E. S., Darnell, S. C., Sanz, P., Rasmussen, S. B., O'Brien, A. D. (2007). Bacillus anthracis Exosporium Protein BclA Affects Spore Germination, Interaction with Extracellular Matrix Proteins, and Hydrophobicity. Infect. Immun. 75: 5233-5239 [Abstract] [Full Text]  
  • Bergman, N. H., Anderson, E. C., Swenson, E. E., Janes, B. K., Fisher, N., Niemeyer, M. M., Miyoshi, A. D., Hanna, P. C. (2007). Transcriptional Profiling of Bacillus anthracis during Infection of Host Macrophages. Infect. Immun. 75: 3434-3444 [Abstract] [Full Text]  
  • Passalacqua, K. D., Bergman, N. H., Lee, J. Y., Sherman, D. H., Hanna, P. C. (2007). The Global Transcriptional Responses of Bacillus anthracis Sterne (34F2) and a {Delta}sodA1 Mutant to Paraquat Reveal Metal Ion Homeostasis Imbalances during Endogenous Superoxide Stress. J. Bacteriol. 189: 3996-4013 [Abstract] [Full Text]  
  • Szurmant, H., Mohan, M. A., Imus, P. M., Hoch, J. A. (2007). YycH and YycI Interact To Regulate the Essential YycFG Two-Component System in Bacillus subtilis. J. Bacteriol. 189: 3280-3289 [Abstract] [Full Text]  
  • Bongiorni, C., Stoessel, R., Perego, M. (2007). Negative Regulation of Bacillus anthracis Sporulation by the Spo0E Family of Phosphatases. J. Bacteriol. 189: 2637-2645 [Abstract] [Full Text]  
  • Lee, J. Y., Janes, B. K., Passalacqua, K. D., Pfleger, B. F., Bergman, N. H., Liu, H., Hakansson, K., Somu, R. V., Aldrich, C. C., Cendrowski, S., Hanna, P. C., Sherman, D. H. (2007). Biosynthetic Analysis of the Petrobactin Siderophore Pathway from Bacillus anthracis. J. Bacteriol. 189: 1698-1710 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»