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Infection and Immunity, March 2006, p. 1989-1993, Vol. 74, No. 3
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.3.1989-1993.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Identification of Secreted Cysteine Proteases from the Parasitic Nematode Haemonchus contortus Detected by Biotinylated Inhibitors

Ana P. Yatsuda,1,2 Nicole Bakker,1 Jeroen Krijgsveld,3 David P. Knox,4 Albert J. R. Heck,3 and Erik de Vries1*

Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil,2 Division of Infection Biology, Department of Infectious Diseases and Immunology,1 Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands,3 Moredun Research Institute, International Research Centre, Pentlands Science Park, Penicuik, United Kingdom4

Received 12 October 2005/ Returned for modification 22 November 2005/ Accepted 18 December 2005

Seven cathepsin B-like cysteine proteases (CBLs) were identified from the immunoprotective excretory-secretory products of Haemonchus contortus. Two-dimensional (2-D) zymography and biotinylated inhibitors were employed to localize active CBLs in 2-D protein gels. Mass spectrometry provided the identification of AC-4, HMCP1, HMCP2, and GCP7 as well as three novel CBLs encoded by clustered expressed sequence tags.

* Corresponding author. Mailing address: Division of Infection Biology, Department of Infectious Diseases and Immunology, Utrecht University, Yalelaan, 1, 3584CL, Utrecht, The Netherlands. Phone: 31-30-2532582. Fax: 31-30-2540784. E-mail: e.devries{at}vet.uu.nl.

Editor: W. A. Petri, Jr.

Infection and Immunity, March 2006, p. 1989-1993, Vol. 74, No. 3
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.3.1989-1993.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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