This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dudley, E. G.
Right arrow Articles by Nataro, J. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dudley, E. G.
Right arrow Articles by Nataro, J. P.

 Previous Article  |  Next Article 

Infection and Immunity, April 2006, p. 2102-2114, Vol. 74, No. 4
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.4.2102-2114.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

An IncI1 Plasmid Contributes to the Adherence of the Atypical Enteroaggregative Escherichia coli Strain C1096 to Cultured Cells and Abiotic Surfaces

Edward G. Dudley,1 Cecilia Abe,5,{dagger} Jean-Marc Ghigo,6 Patricia Latour-Lambert,6 Juan Carlos Hormazabal,7 and James P. Nataro1,2,3,4*

Center for Vaccine Development,1 Departments of Pediatrics,2 Medicine,3 Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland,4 Universidade Federal de Sao Paulo, Sao Paulo, Brazil,5 Groupe de Génétique des Biofilms, Institut Pasteur, URA CNRS 2172, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France,6 Bacteriology Section, Instituto de Salud Publica, Santiago, Chile7

Received 13 September 2005/ Returned for modification 25 October 2005/ Accepted 21 December 2005

Enteroaggregative Escherichia coli (EAEC) is defined by a characteristic "stacked-brick" aggregative adherence (AA) pattern to cultured cells. In well-studied EAEC prototype strains (called typical EAEC strains), the AA phenotype requires aggregative adherence fimbriae (AAFs). However, previous studies suggest that known AAF alleles are not found in all EAEC strains. To define mechanisms contributing to adherence in an atypical strain, we studied EAEC strain C1096. An E. coli K12 derivative carrying two plasmids, designated pSERB1 and pSERB2, from C1096 adhered to cell lines and exhibited an AA pattern. Nucleotide sequence analysis of pSERB1 indicated that it is related to plasmids of the IncI1 incompatibility group. These plasmids encode genes involved in pilus-mediated conjugal transfer, as well as pilL-V, which encodes a second pilus of the type IV family. Insertional inactivation of the gene predicted to encode the major type IV pilin subunit (pilS) reduced conjugal transfer of the plasmid by 4 orders of magnitude. Adherence of the mutant strain to polystyrene and to HT29 cells was reduced by approximately 21% and 75%, respectively. In a continuous-flow microfermentor, the pilS inactivation reduced mature biofilm formation on a glass slide by approximately 50%. In addition, the simultaneous presence of both pSERB1 and pSERB2 plasmids promoted pilS-independent biofilm formation. We conclude that the IncI1 plasmid of EAEC C1096 encodes a type IV pilus that contributes to plasmid conjugation, epithelial cell adherence, and adherence to abiotic surfaces. We also observe that AA can be mediated by factors distinct from AAF adhesins.

* Corresponding author. Mailing address: University of Maryland, 685 W. Baltimore St., Baltimore, MD 21201. Phone: (410) 706 5328. Fax: (410) 706 6205. E-mail: jnataro{at}medicine.umaryland.edu.

Editor: A. D. O'Brien

{dagger} Present address: Institute of Child Health, University of Birmingham, B4 6LH Birmingham, United Kingdom.

Infection and Immunity, April 2006, p. 2102-2114, Vol. 74, No. 4
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.4.2102-2114.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Valverde, A., Canton, R., Garcillan-Barcia, M. P., Novais, A., Galan, J. C., Alvarado, A., de la Cruz, F., Baquero, F., Coque, T. M. (2009). Spread of blaCTX-M-14 Is Driven Mainly by IncK Plasmids Disseminated among Escherichia coli Phylogroups A, B1, and D in Spain. Antimicrob. Agents Chemother. 53: 5204-5212 [Abstract] [Full Text]  
  • Bhargava, S., Johnson, B. B., Hwang, J., Harris, T. A., George, A. S., Muir, A., Dorff, J., Okeke, I. N (2009). Heat-Resistant Agglutinin 1 Is an Accessory Enteroaggregative Escherichia coli Colonization Factor. J. Bacteriol. 191: 4934-4942 [Abstract] [Full Text]  
  • Barros, S. F., Abe, C. M., Rocha, S. P. D., Ruiz, R. M., Beutin, L., Trabulsi, L. R., Elias, W. P. (2008). Escherichia coli O125ac:H6 Encompasses Atypical Enteropathogenic E. coli Strains That Display the Aggregative Adherence Pattern. J. Clin. Microbiol. 46: 4052-4055 [Abstract] [Full Text]  
  • Oshima, K., Toh, H., Ogura, Y., Sasamoto, H., Morita, H., Park, S.-H., Ooka, T., Iyoda, S., Taylor, T. D., Hayashi, T., Itoh, K., Hattori, M. (2008). Complete Genome Sequence and Comparative Analysis of the Wild-type Commensal Escherichia coli Strain SE11 Isolated from a Healthy Adult. DNA Res 15: 375-386 [Abstract] [Full Text]  
  • Burmolle, M., Bahl, M. I., Jensen, L. B., Sorensen, S. J., Hansen, L. H. (2008). Type 3 fimbriae, encoded by the conjugative plasmid pOLA52, enhance biofilm formation and transfer frequencies in Enterobacteriaceae strains. Microbiology 154: 187-195 [Abstract] [Full Text]  
  • Samie, A., Obi, C. L., Dillingham, R., Pinkerton, R. C., Guerrant, R. L. (2007). Enteroaggregative Escherichia coli in Venda, South Africa: Distribution of Virulence-Related Genes by Multiplex Polymerase Chain Reaction in Stool Samples of Human Immunodeficiency Virus (HIV)-Positive and HIV-Negative Individuals and Primary School Children. Am J Trop Med Hyg 77: 142-150 [Abstract] [Full Text]  
  • Joo, L. M., Macfarlane-Smith, L. R., Okeke, I. N. (2007). Error-Prone DNA Repair System in Enteroaggregative Escherichia coli Identified by Subtractive Hybridization. J. Bacteriol. 189: 3793-3803 [Abstract] [Full Text]  
  • Mohamed, J. A., Huang, D. B., Jiang, Z.-D., DuPont, H. L., Nataro, J. P., Belkind-Gerson, J., Okhuysen, P. C. (2007). Association of Putative Enteroaggregative Escherichia coli Virulence Genes and Biofilm Production in Isolates from Travelers to Developing Countries. J. Clin. Microbiol. 45: 121-126 [Abstract] [Full Text]  
  • Jenkins, C., Tembo, M., Chart, H., Cheasty, T., Willshaw, G. A., Phillips, A. D., Tompkins, D., Smith, H. (2006). Detection of enteroaggregative Escherichia coli in faecal samples from patients in the community with diarrhoea.. J Med Microbiol 55: 1493-1497 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»