This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Leone, C. W.
Right arrow Articles by Graves, D. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Leone, C. W.
Right arrow Articles by Graves, D. T.

 Previous Article  |  Next Article 

Infection and Immunity, April 2006, p. 2286-2292, Vol. 74, No. 4
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.4.2286-2292.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Immunization Enhances Inflammation and Tissue Destruction in Response to Porphyromonas gingivalis

Cataldo W. Leone, Haneen Bokhadhoor,{dagger} David Kuo,{dagger} Tesfahun Desta, Julia Yang, Michelle F. Siqueira, Salomon Amar, and Dana T. Graves*

Department of Periodontology and Oral Biology, School of Dental Medicine, Boston University, Boston, Massachusetts

Received 28 June 2005/ Returned for modification 26 July 2005/ Accepted 8 December 2005

It is well established that host-bacterium interactions play a critical role in the initiation and progression of periodontal diseases. By the use of inhibitors, it has been shown that mediators associated with the innate immune response significantly contribute to the disease process. Less is known regarding the role of the acquired immune response. To investigate mechanisms by which the acquired immune response to Porphyromonas gingivalis could affect connective tissue, we used a well-documented calvarial model to study host-bacterium interactions. Injection of P. gingivalis stimulated gamma interferon, interleukin 6, macrophage inflammatory protein 2, and monocyte chemoattractant protein 1 expression as determined by real-time PCR. Prior immunization against P. gingivalis significantly enhanced the mRNA levels of these cytokines and chemokines. Similarly, immunization significantly increased and prolonged the formation of a polymorphonuclear leukocyte and mononuclear cell infiltrate (P < 0.05). In addition, the area of connective tissue destruction, osteoclastogenesis, bone loss, mRNA expression of proapoptotic genes, and degree of fibroblast apoptosis were increased in immunized mice (P < 0.05). These results indicate that activation of the acquired immunity by P. gingivalis increases the inflammatory and destructive responses which occur in part through up-regulating the innate immune response and enhancing osteoclastogenesis and fibroblast apoptosis.

* Corresponding author. Mailing address: Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, 700 Albany Street, W-201, Boston, MA 02118. Phone: (617) 638-4733. Fax: (617) 938-8837. E-mail: dgraves{at}bu.edu.

Editor: J. D. Clements

{dagger} These authors contributed equally to this project.

Infection and Immunity, April 2006, p. 2286-2292, Vol. 74, No. 4
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.4.2286-2292.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Behl, Y., Siqueira, M., Ortiz, J., Li, J., Desta, T., Faibish, D., Graves, D. T. (2008). Activation of the Acquired Immune Response Reduces Coupled Bone Formation in Response to a Periodontal Pathogen. J. Immunol. 181: 8711-8718 [Abstract] [Full Text]  
  • Patil, C.S., Kirkwood, K.L. (2007). p38 MAPK Signaling in Oral-related Diseases. JDR 86: 812-825 [Abstract] [Full Text]  
  • Stashenko, P., Goncalves, R. B., Lipkin, B., Ficarelli, A., Sasaki, H., Campos-Neto, A. (2007). Th1 Immune Response Promotes Severe Bone Resorption Caused by Porphyromonas gingivalis. Am. J. Pathol. 170: 203-213 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»