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Infection and Immunity, April 2006, p. 2436-2441, Vol. 74, No. 4
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.4.2436-2441.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

MyD88 Innate Immune Function in a Zebrafish Embryo Infection Model

Astrid M. van der Sar,1 Oliver W. Stockhammer,2 Carina van der Laan,1 Herman P. Spaink,2 Wilbert Bitter,1 and Annemarie H. Meijer2*

Department of Medical Microbiology, VU Medical Centre, Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands,1 Institute of Biology, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands2

Received 5 August 2005/ Returned for modification 4 October 2005/ Accepted 11 January 2006

Innate immunity signaling mechanisms during vertebrate embryogenesis are largely unknown. To study Toll-like receptor (TLR) signaling function in the zebrafish embryo model, we designed an experimental setup for antisense morpholino knockdown under conditions of bacterial infection. Clearance of Salmonella enterica serovar Typhimurium Ra bacteria was significantly impaired after knockdown of myeloid differentiation factor 88 (MyD88), a common adaptor protein in TLR and interleukin-1 receptor signaling. Thereby, we demonstrate for the first time that the innate immune response of the developing embryo involves MyD88-dependent signaling, which further establishes the zebrafish embryo as a model for the study of vertebrate innate immunity.

* Corresponding author. Mailing address: Institute of Biology, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands. Phone: 31 71 5274927. Fax: 31 71 5274999. E-mail: meijer{at}rulbim.leidenuniv.nl.

Editor: J. L. Flynn

Infection and Immunity, April 2006, p. 2436-2441, Vol. 74, No. 4
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.4.2436-2441.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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