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Infection and Immunity, May 2006, p. 2697-2705, Vol. 74, No. 5
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.5.2697-2705.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Campylobacter jejuni Induces Maturation and Cytokine Production in Human Dendritic Cells

Lan Hu, Mechelle D. Bray, Manuel Osorio, and Dennis J. Kopecko*

Laboratory of Enteric and Sexually Transmitted Diseases, Center for Biologics Evaluation and Research, Food and Drug Administration, 29 Lincoln Drive, NIH Campus, Building 29/420, HFM440, Bethesda, Maryland 20892

Received 28 October 2005/ Returned for modification 27 November 2005/ Accepted 1 February 2006

Campylobacter jejuni is a leading bacterial cause of human diarrheal disease in both developed and developing nations. Colonic mucosal invasion and the resulting host inflammatory responses are thought to be the key contributing factors to the dysenteric form of this disease. Dendritic cells (DCs) play an important role in both the innate and adaptive immune responses to microbial infection. In this study, the interaction between human monocyte-derived dendritic cells and C. jejuni was studied. We found that C. jejuni was readily internalized by DCs over a 2-h period. However, after a prolonged infection period (24 or 48 h) with C. jejuni, only a few viable bacteria remained intracellularly. Minimal cytotoxicity of C. jejuni to dendritic cells was observed. C. jejuni induced the maturation of dendritic cells over 24 h, as indicated by up-regulation of cell surface marker proteins CD40, CD80, and CD86. In addition, Campylobacter-infected DCs triggered activation of NF-{kappa}B and significantly stimulated production of interleukin-1ß (IL-1ß), IL-6, IL-8, IL-10, IL-12, gamma interferon, and tumor necrosis factor alpha (TNF-{alpha}) compared to uninfected DCs. Active bacterial invasion of DCs was not necessary for the induction of these cytokines, as heat-killed C. jejuni stimulated similar levels of cytokine production as live bacteria. Purified lipooligosaccharide of C. jejuni appears to be the major stimulant for the increased production of cytokines by DCs. Taken together, these data indicate that during infection, Campylobacter triggers an innate inflammatory response through increased production of IL-1ß, IL-6, IL-8, and TNF-{alpha} and initiates a Th1-polarized adaptive immune response as predicted from the high level of production of IL-12.

* Corresponding author. Mailing address: Laboratory of Enteric and Sexually Transmitted Diseases, Center for Biologics Evaluation and Research, Food and Drug Administration, 29 Lincoln Drive, NIH Campus, Bldg. 29/420, HFM440, Bethesda, MD 20892. Phone: (301) 496-1893. Fax: (301) 402-8701. E-mail: kopecko{at}cber.fda.gov.

Editor: J. T. Barbieri

Infection and Immunity, May 2006, p. 2697-2705, Vol. 74, No. 5
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.5.2697-2705.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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