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Infection and Immunity, May 2006, p. 2706-2716, Vol. 74, No. 5
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.5.2706-2716.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Safety, Immunogenicity, and Efficacy of Prime-Boost Immunization with Recombinant Poxvirus FP9 and Modified Vaccinia Virus Ankara Encoding the Full-Length Plasmodium falciparum Circumsporozoite Protein

Centre for Clinical Vaccinology & Tropical Medicine, Nuffield Department of Clinical Medicine, Oxford University, Churchill Hospital, Oxford OX3 7LJ, United Kingdom,¹ The Wellcome Trust Centre for Human Genetics, Nuffield Department of Clinical Medicine, Oxford University, Roosevelt Drive, Oxford OX3 7BN, United Kingdom,² PATH Malaria Vaccine Initiative, Bethesda, Maryland 20814,³ Department of Biological Sciences, Sir Alexander Fleming Building, Imperial College London, Imperial College Road, London SW7 2AZ, United Kingdom,⁴ Walter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, Maryland 20910⁵

Received 15 December 2005/ Returned for modification 3 February 2006/ Accepted 25 February 2006

Heterologous prime-boost immunization with DNA and various recombinant poxviruses encoding malaria antigens is capable of inducing strong cell-mediated immune responses and partial protection in human sporozoite challenges. Here we report a series of trials assessing recombinant fowlpox virus and modified vaccinia virus Ankara encoding the Plasmodium falciparum circumsporozoite protein in various prime-boost combinations, doses, and application routes. For the first time, these vaccines were administered intramuscularly and at doses of up to 5 x 10⁸ PFU. Vaccines containing this antigen proved safe and induced modest immune responses but showed no evidence of efficacy in a sporozoite challenge.

Editor: W. A. Petri, Jr.

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