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Infection and Immunity, May 2006, p. 3030-3034, Vol. 74, No. 5
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.5.3030-3034.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Evidence that the BBA68 Protein (BbCRASP-1) of the Lyme Disease Spirochetes Does Not Contribute to Factor H-Mediated Immune Evasion in Humans and Other Animals

John V. McDowell,1 Kelley M. Hovis,1 Hongming Zhang,1 Emily Tran,1 Justin Lankford,1 and R. T. Marconi1,2*

Department of Microbiology and Immunology,1 Center for the Study of Biological Complexity, Medical College of Virginia at Virginia Commonwealth University, Richmond, Virginia 23298-06782

Received 13 October 2005/ Returned for modification 29 November 2005/ Accepted 17 February 2006

BBA68 (BbCRASP-1) of the Lyme disease spirochetes binds human factor H (FH) and FH-like protein 1 (FHL-1). Here we assess transcription of the BBA68 gene and production of BBA68 in infected mice and humans using real-time reverse transcriptase PCR and immunoblotting. The species specificity of FH binding to BBA68 was also tested. The data suggest that BBA68 does not play an important role in immune evasion in animals.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Medical College of Virginia at Virginia Commonwealth University, Richmond, VA 23298-0678. Phone: (804) 828-3779. Fax: (804) 828-9946. E-mail: rmarconi{at}hsc.vcu.edu.

Editor: J. B. Bliska

Infection and Immunity, May 2006, p. 3030-3034, Vol. 74, No. 5
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.5.3030-3034.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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