This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nossa, C. W.
Right arrow Articles by Blanke, S. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nossa, C. W.
Right arrow Articles by Blanke, S. R.

 Previous Article  |  Next Article 

Infection and Immunity, May 2006, p. 3071-3076, Vol. 74, No. 5
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.5.3071-3076.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Modification of a Mammalian Cell Protein in the Presence of [32P-Adenylate]NAD: Evidence for ADP Ribosylation Activity Associated with Helicobacter pylori

Carlos W. Nossa and Steven R. Blanke*

Department of Microbiology, University of Illinois, Urbana, Illinois 61801Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204-5001

Received 19 November 2005/ Returned for modification 24 December 2005/ Accepted 24 February 2006

Culture filtrates from Helicobacter pylori promote the transfer of the radiolabel from [32P-adenylate]NAD to one or more heat-labile factors within extracts prepared from several mammalian cell lines, with the predominate radiolabeled species exhibiting an apparent molecular mass of greater than 130 kDa. Our results suggest that several H. pylori strains release a factor that ADP-ribosylates a mammalian target protein.


* Corresponding author. Mailing address: Department of Microbiology, B103 CLSL, University of Illinois, 601 South Goodwin Ave., Urbana, IL 61801. Phone: (217) 244-2412. Fax: (713) 743-8351. E-mail: sblanke{at}life.uiuc.edu.

Editor: D. L. Burns


Infection and Immunity, May 2006, p. 3071-3076, Vol. 74, No. 5
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.5.3071-3076.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.