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Infection and Immunity, May 2006, p. 3071-3076, Vol. 74, No. 5
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.5.3071-3076.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Modification of a Mammalian Cell Protein in the Presence of [32P-Adenylate]NAD: Evidence for ADP Ribosylation Activity Associated with Helicobacter pylori

Carlos W. Nossa and Steven R. Blanke*

Department of Microbiology, University of Illinois, Urbana, Illinois 61801Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204-5001

Received 19 November 2005/ Returned for modification 24 December 2005/ Accepted 24 February 2006

Culture filtrates from Helicobacter pylori promote the transfer of the radiolabel from [32P-adenylate]NAD to one or more heat-labile factors within extracts prepared from several mammalian cell lines, with the predominate radiolabeled species exhibiting an apparent molecular mass of greater than 130 kDa. Our results suggest that several H. pylori strains release a factor that ADP-ribosylates a mammalian target protein.

* Corresponding author. Mailing address: Department of Microbiology, B103 CLSL, University of Illinois, 601 South Goodwin Ave., Urbana, IL 61801. Phone: (217) 244-2412. Fax: (713) 743-8351. E-mail: sblanke{at}life.uiuc.edu.

Editor: D. L. Burns

Infection and Immunity, May 2006, p. 3071-3076, Vol. 74, No. 5
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.5.3071-3076.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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