This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Johanesen, P. A.
Right arrow Articles by Dwinell, M. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Johanesen, P. A.
Right arrow Articles by Dwinell, M. B.

 Previous Article  |  Next Article 

Infection and Immunity, June 2006, p. 3437-3447, Vol. 74, No. 6
0019-9567/06/$08.00+0     doi:10.1128/IAI.01740-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Flagellin-Independent Regulation of Chemokine Host Defense in Campylobacter jejuni-Infected Intestinal Epithelium

Priscilla A. Johanesen and Michael B. Dwinell*

Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226

Received 27 October 2005/ Returned for modification 30 December 2005/ Accepted 22 March 2006

Campylobacter jejuni is a leading cause of bacterial food-borne diarrheal disease throughout the world and the most frequent antecedent of autoimmune neuropathy Guillain-Barré syndrome. While infection is associated with immune memory, little is known regarding the role of the epithelium in targeting dendritic cells (DC) for initiating the appropriate adaptive immune response to C. jejuni. The objective of this study was to define the role for the intestinal epithelium in the induction of the adaptive immune response in C. jejuni infection by assessing the production of DC and T-cell chemoattractants. Human T84 epithelial cells were used as model intestinal epithelia. Infection of T84 cells with C. jejuni dose- and time-dependently up-regulated DC and T-cell chemokine gene transcription and secretion. Induction required live bacteria and was in the physiologically relevant direction for attraction of mucosal immunocytes. C. jejuni-activated NF-{kappa}B signaling was shown to be essential for proinflammatory chemokine secretion. Notably, C. jejuni secretion occurred independently of flagellin identification by Toll-like receptor 5. Secretion of a DC chemoattractant by differing clinical C. jejuni isolates suggested adherence/invasion were key virulence determinants of epithelial chemokine secretion. The regulated epithelial expression of DC and T-cell chemoattractants suggests a mechanism for the directed trafficking of immune cells required for the initiation of adaptive immunity in campylobacteriosis. Chemokine secretion occurs despite Campylobacter evasion of the flagellin pattern recognition receptor, suggesting that alternate host defense strategies limit disease pathogenesis.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226. Phone: (414) 456-7427. Fax: (414) 456-6535. E-mail: mdwinell{at}mcw.edu.

Editor: V. J. DiRita

Infection and Immunity, June 2006, p. 3437-3447, Vol. 74, No. 6
0019-9567/06/$08.00+0     doi:10.1128/IAI.01740-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Winter, S. E., Thiennimitr, P., Nuccio, S.-P., Haneda, T., Winter, M. G., Wilson, R. P., Russell, J. M., Henry, T., Tran, Q. T., Lawhon, S. D., Gomez, G., Bevins, C. L., Russmann, H., Monack, D. M., Adams, L. G., Baumler, A. J. (2009). Contribution of Flagellin Pattern Recognition to Intestinal Inflammation during Salmonella enterica Serotype Typhimurium Infection. Infect. Immun. 77: 1904-1916 [Abstract] [Full Text]  
  • Friis, L. M., Keelan, M., Taylor, D. E. (2009). Campylobacter jejuni Drives MyD88-Independent Interleukin-6 Secretion via Toll-Like Receptor 2. Infect. Immun. 77: 1553-1560 [Abstract] [Full Text]  
  • Larson, C. L., Shah, D. H., Dhillon, A. S., Call, D. R., Ahn, S., Haldorson, G. J., Davitt, C., Konkel, M. E. (2008). Campylobacter jejuni invade chicken LMH cells inefficiently and stimulate differential expression of the chicken CXCLi1 and CXCLi2 cytokines. Microbiology 154: 3835-3847 [Abstract] [Full Text]  
  • Zheng, J., Meng, J., Zhao, S., Singh, R., Song, W. (2008). Campylobacter-Induced Interleukin-8 Secretion in Polarized Human Intestinal Epithelial Cells Requires Campylobacter-Secreted Cytolethal Distending Toxin- and Toll-Like Receptor-Mediated Activation of NF-{kappa}B. Infect. Immun. 76: 4498-4508 [Abstract] [Full Text]  
  • Steiner, T. S. (2007). How Flagellin and Toll-Like Receptor 5 Contribute to Enteric Infection. Infect. Immun. 75: 545-552 [Full Text]  
  • Chen, M. L., Ge, Z., Fox, J. G., Schauer, D. B. (2006). Disruption of Tight Junctions and Induction of Proinflammatory Cytokine Responses in Colonic Epithelial Cells by Campylobacter jejuni. Infect. Immun. 74: 6581-6589 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»