This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kaparakis, M.
Right arrow Articles by Strugnell, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kaparakis, M.
Right arrow Articles by Strugnell, R. A.

 Previous Article  |  Next Article 

Infection and Immunity, June 2006, p. 3519-3529, Vol. 74, No. 6
0019-9567/06/$08.00+0     doi:10.1128/IAI.01314-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

CD4+ CD25+ Regulatory T Cells Modulate the T-Cell and Antibody Responses in Helicobacter-Infected BALB/c Mice

Maria Kaparakis,1,{dagger} Karen L. Laurie,2,{dagger} Odilia Wijburg,1,3 John Pedersen,4 Martin Pearse,5 Ian R. van Driel,2,{ddagger} Paul A. Gleeson,2,{ddagger}* and Richard A. Strugnell1,3,{ddagger}

Department of Microbiology and Immunology and the NHMRC Bacterial Pathogenesis Group, The University of Melbourne, Parkville, Victoria 3010, Australia,1 Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Victoria 3010, Australia,2 CRC for Vaccine Technology, Brisbane, Australia,3 Department of Anatomical Pathology, Alfred Hospital, Prahran, Victoria 3181, Australia,4 CSL Ltd., Poplar Rd., Parkville, Victoria 3052, Australia5

Received 12 August 2005/ Returned for modification 17 November 2005/ Accepted 6 March 2006

Gastric Helicobacter spp. induce chronic gastritis that may lead to ulceration and dysplasia. The host elicits a T helper 1 (Th1) response that is fundamental to the pathogenesis of these bacteria. We analyzed immune responses in Helicobacter-infected, normal mice depleted of CD4+ CD25+ T cells to investigate the in vivo role of regulatory T cells (Tregs) in the modulation of Helicobacter immunopathology. BALB/c and transgenic mice were depleted of CD4+ CD25+ T cells by administration of an anti-CD25 antibody either at the time of infection with Helicobacter or during chronic infection and gastritis. Depletion of CD25+ Tregs prior to and during infection of mice with Helicobacter spp. did not affect either bacterial colonization or severity of gastritis. Depletion of CD25+ Tregs was associated with increased Helicobacter-specific antibody levels and an altered isotype distribution. Paragastric lymph node cells from CD25+ Treg-depleted and control infected mice showed similar proliferation to Helicobacter antigens, but only cells from anti-CD25-treated animals secreted Th2 cytokines. CD25+ Tregs do not control the level of gastritis induced by gastric Helicobacter spp. in normal, thymus-intact BALB/c mice. However, CD25+ Tregs influence the cytokine and antibody responses induced by infection. Autoimmune gastritis is not induced in Helicobacter-infected mice depleted of CD25+ Tregs but is induced in CD25+ Treg-depleted mice, which have a higher frequency of autoreactive T cells.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Victoria 3010, Australia. Phone: 61 3 8344 2354. Fax: 61 3 9348 1421. E-mail: pgleeson{at}unimelb.edu.au.

Editor: F. C. Fang

{dagger} M.K. and K.L.L. contributed equally to this work as first authors.

{ddagger} I.R.V.D., P.A.G., and R.A.S. contributed equally to this work as senior authors.

Infection and Immunity, June 2006, p. 3519-3529, Vol. 74, No. 6
0019-9567/06/$08.00+0     doi:10.1128/IAI.01314-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kaparakis, M., Walduck, A. K., Price, J. D., Pedersen, J. S., van Rooijen, N., Pearse, M. J., Wijburg, O. L. C., Strugnell, R. A. (2008). Macrophages Are Mediators of Gastritis in Acute Helicobacter pylori Infection in C57BL/6 Mice. Infect. Immun. 76: 2235-2239 [Abstract] [Full Text]  
  • Tan, M. P., Pedersen, J., Zhan, Y., Lew, A. M., Pearse, M. J., Wijburg, O. L. C., Strugnell, R. A. (2008). CD8+ T Cells Are Associated with Severe Gastritis in Helicobacter pylori- Infected Mice in the Absence of CD4+ T Cells. Infect. Immun. 76: 1289-1297 [Abstract] [Full Text]  
  • Tan, M. P., Kaparakis, M., Galic, M., Pedersen, J., Pearse, M., Wijburg, O. L. C., Janssen, P. H., Strugnell, R. A. (2007). Chronic Helicobacter pylori Infection Does Not Significantly Alter the Microbiota of the Murine Stomach. Appl. Environ. Microbiol. 73: 1010-1013 [Abstract] [Full Text]  
  • McKinley, L., Logar, A. J., McAllister, F., Zheng, M., Steele, C., Kolls, J. K. (2006). Regulatory T Cells Dampen Pulmonary Inflammation and Lung Injury in an Animal Model of Pneumocystis Pneumonia. J. Immunol. 177: 6215-6226 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»