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Infection and Immunity, June 2006, p. 3682-3683, Vol. 74, No. 6
0019-9567/06/$08.00+0     doi:10.1128/IAI.01731-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Isolation of a Human Monoclonal Antibody with Strong Neutralizing Activity against Diphtheria Toxin

Mai Kakita,1 Tsuyoshi Takahashi,2 Takako Komiya,3 Yoshitaka Iba,4 Takao Tsuji,2 Yoshikazu Kurosawa,4* and Motohide Takahashi3

Institute for Antibodies Ltd.,1 Department of Microbiology,2 School of Medicine, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi 470-1192,4 National Institute of Infectious Diseases, Musashimurayama, Tokyo 208-0011, Japan3

Received 21 October 2005/ Returned for modification 1 November 2005/ Accepted 29 March 2006

We isolated a human monoclonal antibody against diphtheria toxin (DT). It bound to fragment B with a binding activity (Kd) of 3.01 nM. The neutralizing activity assayed by the rabbit skin test was estimated to be 73,600 IU/g. This could be used as a therapeutic drug against DT in place of the traditional equine sera.

* Corresponding author. Mailing address: Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi 470-1192, Japan. Phone: 81-562-93-9387. Fax: 82-562-93-8835. E-mail: kurosawa{at}fujita-hu.ac.jp.

Editor: J. B. Bliska

Infection and Immunity, June 2006, p. 3682-3683, Vol. 74, No. 6
0019-9567/06/$08.00+0     doi:10.1128/IAI.01731-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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