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Infection and Immunity, June 2006, p. 3687-3691, Vol. 74, No. 6
0019-9567/06/$08.00+0     doi:10.1128/IAI.01837-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The ABC Transporter Protein OppA Provides Protection against Experimental Yersinia pestis Infection

Mikio Tanabe,1,2 Helen S. Atkins,3 David N. Harland,3 Stephen J. Elvin,3 Anthony J. Stagg,3 Osman Mirza,2 Richard W. Titball,3,4* Bernadette Byrne,2 and Katherine A. Brown1

Division of Cell and Molecular Biology, Centre for Molecular Microbiology and Infection, Imperial College London, London SW7 2AZ, United Kingdom,1 Division of Molecular Biosciences, Membrane Protein Crystallography Group, Imperial College London, London SW7 2AZ, United Kingdom,2 Defence Science and Technology Laboratory, Porton Down, Salisbury SP4 0JQ, United Kingdom,3 Department of Infectious and Tropical Disease, London School of Hygiene and Tropical Medicine, Keppel St., London, WC1E 7HT, United Kingdom4

Received 10 November 2005/ Returned for modification 21 December 2005/ Accepted 16 March 2006

The identification of Yersinia pestis as a potential bioterrorism agent and the emergence of antibiotic-resistant strains have highlighted the need for improved vaccines and treatments for plague. The aim of this study was to evaluate the potential for ATP-binding cassette (ABC) transporter proteins to be exploited as novel vaccines against plague. Western blotting of ABC transporter proteins using sera from rabbits immunized with killed whole Y. pestis cells or human convalescent-phase sera identified four immunologically reactive proteins: OppA, PstS, YrbD, and PiuA. Mice immunized with these proteins developed antibody to the immunogen. When the immunized mice were challenged with Y. pestis, the OppA-immunized mice showed an increased time to death compared to other groups, and protection appeared to correlate with the level of immunoglobulin G antibody to OppA.

* Corresponding author. Mailing address: Defence Science and Technology Laboratory, Porton Down, Salisbury SP4 0JQ, United Kingdom. Phone: 44 20 7594 3004. Fax: 44 1980 614307. E-mail: rtitball{at}dstl.gov.uk.

Editor: J. B. Bliska

Infection and Immunity, June 2006, p. 3687-3691, Vol. 74, No. 6
0019-9567/06/$08.00+0     doi:10.1128/IAI.01837-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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