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Infection and Immunity, July 2006, p. 3967-3978, Vol. 74, No. 7
0019-9567/06/$08.00+0     doi:10.1128/IAI.01128-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Circulating {gamma}{delta} T Cells in Response to Salmonella enterica Serovar Enteritidis Exposure in Chickens

Angela Berndt,1* Jana Pieper,2 and Ulrich Methner2

Institute of Molecular Pathogenesis,1 Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institute, Naumburger Str. 96a, D-07743 Jena, Germany2

Received 15 July 2005/ Returned for modification 12 October 2005/ Accepted 4 April 2006

{gamma}{delta} T cells are considered crucial to the outcome of various infectious diseases. The present study was undertaken to characterize {gamma}{delta} (T-cell receptor 1+ [TCR1+]) T cells phenotypically and functionally in avian immune response. Day-old chicks were orally immunized with Salmonella enterica serovar Enteritidis live vaccine or S. enterica serovar Enteritidis wild-type strain and infected using the S. enterica serovar Enteritidis wild-type strain on day 44 of life. Between days 3 and 71, peripheral blood was examined flow cytometrically for the occurrence of {gamma}{delta} T-cell subpopulations differentiated by the expression of T-cell antigens. Three different TCR1+ cell populations were found to display considerable variation regarding CD8{alpha} antigen expression: (i) CD8{alpha}+high TCR1+ cells, (ii) CD8{alpha}+dim TCR1+ cells, and (iii) CD8{alpha}– TCR1+ cells. While most of the CD8{alpha}+high TCR1+ cells expressed the CD8{alpha}ß heterodimeric antigen, the majority of the CD8{alpha}+dim TCR1+ cells were found to express the CD8{alpha}{alpha} homodimeric form. After immunization, a significant increase of CD8{alpha}{alpha}+high {gamma}{delta} T cells was observed within the CD8{alpha}+high TCR1+ cell population. Quantitative reverse transcription-PCR revealed reduced interleukin-7 receptor {alpha} (IL-7R{alpha}) and Bcl-x expression and elevated IL-2R{alpha} mRNA expression of the CD8{alpha}{alpha}+high {gamma}{delta} T cells. Immunohistochemical analysis demonstrated a significant increase of CD8{alpha}+ and TCR1+ cells in the cecum and spleen and a decreased percentage of CD8ß+ T cells in the spleen after Salmonella immunization. After infection of immunized animals, immune reactions were restricted to intestinal tissue. The study showed that Salmonella immunization of very young chicks is accompanied by an increase of CD8{alpha}{alpha}+high {gamma}{delta} T cells in peripheral blood, which are probably activated, and thus represent an important factor for the development of a protective immune response to Salmonella organisms in chickens.

* Corresponding author. Mailing address: Friedrich-Loeffler-Institute, Institute of Molecular Pathogenesis, Naumburger Str. 96a, D-07743 Jena, Germany. Phone: 49 3641 804 410. Fax: 49 3641 804 228. E-mail: angela.berndt{at}fli.bund.de.

Editor: F. C. Fang

Infection and Immunity, July 2006, p. 3967-3978, Vol. 74, No. 7
0019-9567/06/$08.00+0     doi:10.1128/IAI.01128-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Li, H., Zhang, Y., Zuo, S. F., Lian, Z. X., Li, N. (2009). Effects of methyltestosterone on immunity against Salmonella Pullorum in dwarf chicks. Poult. Sci. 88: 2539-2548 [Abstract] [Full Text]  
  • Berndt, A., Wilhelm, A., Jugert, C., Pieper, J., Sachse, K., Methner, U. (2007). Chicken Cecum Immune Response to Salmonella enterica Serovars of Different Levels of Invasiveness. Infect. Immun. 75: 5993-6007 [Abstract] [Full Text]  


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