This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lee, B.-Y.
Right arrow Articles by Clemens, D. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, B.-Y.
Right arrow Articles by Clemens, D. L.

 Previous Article  |  Next Article 

Infection and Immunity, July 2006, p. 4002-4013, Vol. 74, No. 7
0019-9567/06/$08.00+0     doi:10.1128/IAI.00257-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Identification, Recombinant Expression, Immunolocalization in Macrophages, and T-Cell Responsiveness of the Major Extracellular Proteins of Francisella tularensis

Bai-Yu Lee, Marcus A. Horwitz, and Daniel L. Clemens*

Division of Infectious Diseases, Department of Medicine, Center for Health Sciences, University of California, Los Angeles, School of Medicine, Los Angeles, California 90095-1688

Received 16 February 2006/ Returned for modification 7 April 2006/ Accepted 24 April 2006

A safer and more effective vaccine than the previously developed live attenuated vaccine is needed for combating Francisella tularensis, a highly infectious bacterial pathogen. To search for potential candidates for inclusion in a new vaccine, we characterized the proteins present in the culture filtrates of a virulent recent clinical isolate and the attenuated live vaccine strain of F. tularensis using a proteomic approach. We identified a total of 12 proteins; among these, catalase-peroxidase was much more abundant in the culture filtrate of the virulent clinical isolate, whereas bacterioferritin was more abundant in the culture filtrate of the live vaccine strain. Streptolysin O treatment of infected human macrophages indicated that catalase-peroxidase and the heat shock protein GroEL are released intracellularly by actively growing F. tularensis. Mice immunized with F. tularensis developed significant cell-mediated immune responses to catalase-peroxidase, the heat shock protein GroEL, and bacterioferritin as measured by splenic lymphocyte proliferation and gamma interferon production. Finally, we expressed the major culture filtrate proteins that are promising vaccine candidates in Escherichia coli at high levels in soluble form to facilitate study of their immunobiology and potential role in vaccines.

* Corresponding author. Mailing address: Division of Infectious Diseases, 37-121 Center for the Health Sciences, University of California, Los Angeles, 10833 Le Conte Avenue, Los Angeles, CA 90095-1688. Phone: (310) 825-9324. Fax: (310) 793-7156. E-mail: dclemens{at}mednet.ucla.edu.

Editor: D. L. Burns

Infection and Immunity, July 2006, p. 4002-4013, Vol. 74, No. 7
0019-9567/06/$08.00+0     doi:10.1128/IAI.00257-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Clemens, D. L., Lee, B.-Y., Horwitz, M. A. (2009). Francisella tularensis Phagosomal Escape Does Not Require Acidification of the Phagosome. Infect. Immun. 77: 1757-1773 [Abstract] [Full Text]  
  • Savitt, A. G., Mena-Taboada, P., Monsalve, G., Benach, J. L. (2009). Francisella tularensis Infection-Derived Monoclonal Antibodies Provide Detection, Protection, and Therapy. CVI 16: 414-422 [Abstract] [Full Text]  
  • Hazlett, K. R. O., Caldon, S. D., McArthur, D. G., Cirillo, K. A., Kirimanjeswara, G. S., Magguilli, M. L., Malik, M., Shah, A., Broderick, S., Golovliov, I., Metzger, D. W., Rajan, K., Sellati, T. J., Loegering, D. J. (2008). Adaptation of Francisella tularensis to the Mammalian Environment Is Governed by Cues Which Can Be Mimicked In Vitro. Infect. Immun. 76: 4479-4488 [Abstract] [Full Text]  
  • Oyston, P. C. F. (2008). Francisella tularensis: unravelling the secrets of an intracellular pathogen. J Med Microbiol 57: 921-930 [Abstract] [Full Text]  
  • Wuppermann, F. N., Molleken, K., Julien, M., Jantos, C. A., Hegemann, J. H. (2008). Chlamydia pneumoniae GroEL1 Protein Is Cell Surface Associated and Required for Infection of HEp-2 Cells. J. Bacteriol. 190: 3757-3767 [Abstract] [Full Text]  
  • Maier, T. M., Casey, M. S., Becker, R. H., Dorsey, C. W., Glass, E. M., Maltsev, N., Zahrt, T. C., Frank, D. W. (2007). Identification of Francisella tularensis Himar1-Based Transposon Mutants Defective for Replication in Macrophages. Infect. Immun. 75: 5376-5389 [Abstract] [Full Text]  
  • Guina, T., Radulovic, D., Bahrami, A. J., Bolton, D. L., Rohmer, L., Jones-Isaac, K. A., Chen, J., Gallagher, L. A., Gallis, B., Ryu, S., Taylor, G. K., Brittnacher, M. J., Manoil, C., Goodlett, D. R. (2007). MglA Regulates Francisella tularensis subsp. novicida (Francisella novicida) Response to Starvation and Oxidative Stress. J. Bacteriol. 189: 6580-6586 [Abstract] [Full Text]  
  • Lindgren, H., Shen, H., Zingmark, C., Golovliov, I., Conlan, W., Sjostedt, A. (2007). Resistance of Francisella tularensis Strains against Reactive Nitrogen and Oxygen Species with Special Reference to the Role of KatG. Infect. Immun. 75: 1303-1309 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»