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Infection and Immunity, July 2006, p. 4357-4360, Vol. 74, No. 7
0019-9567/06/$08.00+0     doi:10.1128/IAI.01617-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Baculovirus-Expressed Constructs Induce Immunoglobulin G That Recognizes VAR2CSA on Plasmodium falciparum- Infected Erythrocytes

Lea Barfod, Morten A. Nielsen, Louise Turner, Madeleine Dahlbäck, Anja T. R. Jensen, Lars Hviid, Thor G. Theander, and Ali Salanti^*

Centre for Medical Parasitology at Copenhagen University Hospital (Rigshospitalet) and Institute for Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark

Received 4 October 2005/ Returned for modification 3 January 2006/ Accepted 31 March 2006

We raised specific antisera against recombinant VAR2CSA domains produced in Escherichia coli and in insect cells. All were reactive in enzyme-linked immunosorbent assay, but only insect cell-derived constructs induced immunoglobulin G (IgG) that was reactive with native VAR2CSA on the surface of infected erythrocytes. Our data show that five of the six VAR2CSA Duffy-binding-like domains are surface exposed and that induction of surface-reactive VAR2CSA-specific IgG depends critically upon antigen conformation. These findings have implications for the development of vaccines against pregnancy-associated Plasmodium falciparum malaria.

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* Corresponding author. Mailing address: Institute for Medical Microbiology and Immunology, Panum Institute 24-2, Blegdamsvej 3, 2200 Copenhagen N, Denmark. Phone: 45 35 32 76 76. Fax: 45 35 32 78 51. E-mail: salanti{at}cmp.dk.

Editor: J. F. Urban, Jr.

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Infection and Immunity, July 2006, p. 4357-4360, Vol. 74, No. 7
0019-9567/06/$08.00+0     doi:10.1128/IAI.01617-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

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