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Infection and Immunity, July 2006, p. 4383-4386, Vol. 74, No. 7
0019-9567/06/$08.00+0     doi:10.1128/IAI.00429-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Stabilization of a Plasmid Coding for a Heterologous Antigen in Salmonella enterica Serotype Typhi Vaccine Strain CVD908-htrA by Using Site-Specific Recombination

Jonathan C. Stephens,{dagger} Michael J. Darsley,{ddagger} and Arthur K. Turner*

Acambis, Peterhouse Technology Park, 100 Fulbourn Road, Cambridge CB1 9PT, United Kingdom

Received 16 March 2006/ Returned for modification 18 April 2006/ Accepted 24 April 2006

A gene cassette incorporating the crs-rsd site-specific recombination system from the Salmonella enterica subsp. enterica serovar Dublin virulence plasmid improved the inheritance in S. enterica serotype Typhi strain CVD908-htrA of a multicopy plasmid expression vector. Use of this recombination cassette may improve expression of heterologous antigens from multicopy plasmid expression vectors in attenuated bacterial vaccine strains.

* Corresponding author. Present address: The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom. Phone: (44) 1223 834244. Fax: (44) 1223 494919. E-mail: akt{at}sanger.ac.uk.

Editor: D. L. Burns

{dagger} Present address: University of Cambridge, Division of Transfusion Medicine, Department of Haematology, Long Road, Cambridge CB2 2PT, United Kingdom.

{ddagger} Present address: ACE BioSciences A/S, Unsbjergvej 2A, DK-5220 Odense SØ, Denmark.

Infection and Immunity, July 2006, p. 4383-4386, Vol. 74, No. 7
0019-9567/06/$08.00+0     doi:10.1128/IAI.00429-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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