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Infection and Immunity, August 2006, p. 4605-4614, Vol. 74, No. 8
0019-9567/06/$08.00+0     doi:10.1128/IAI.00477-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Maltodextrin Utilization Plays a Key Role in the Ability of Group A Streptococcus To Colonize the Oropharynx

Samuel A. Shelburne III,1 Paul Sumby,2 Izabela Sitkiewicz,2 Nnaja Okorafor,1 Chanel Granville,2 Payal Patel,2 Jovanka Voyich,3 Richard Hull,4 Frank R. DeLeo,3 and James M. Musser2*

Section of Infectious Diseases, Department of Medicine, Baylor College of Medicine, Houston, Texas 77030,1 Center for Molecular and Translational Human Infectious Diseases Research, The Methodist Hospital Research Institute, Houston, Texas 77030,2 Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840,3 Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas 770304

Received 23 March 2006/ Returned for modification 1 May 2006/ Accepted 19 May 2006

Analysis of multiple group A Streptococcus (GAS) genomes shows that genes encoding proteins involved in carbohydrate utilization comprise some 15% of the core GAS genome. Yet there is a limited understanding of how carbohydrate utilization contributes to GAS pathogenesis. Previous genome-wide GAS studies led us to a focused investigation of MalE, a putative maltodextrin-binding protein. Analysis of 28 strains of 22 distinct M protein serotypes showed that MalE is highly conserved among diverse GAS strains. malE transcript levels were significantly increased during growth in human saliva compared to growth in a chemically defined glucose-containing medium or a nutrient-rich medium. MalE was accessible to antibody binding, indicating that it is expressed on the GAS cell surface. Moreover, growth in human saliva appeared to increase MalE surface expression compared to growth in a nutrient-rich medium. Analysis of a {Delta}malE isogenic mutant strain revealed decreased growth in human saliva compared to wild-type GAS. Radiolabeled carbohydrate binding assays showed that MalE was required for the binding of maltose but not glucose. The {Delta}malE isogenic mutant strain colonized a lower percentage of GAS-challenged mice compared to wild-type and genetically complemented strains. Furthermore, decreased numbers of CFU were recovered from mice infected with the {Delta}malE strain compared to those infected with wild-type GAS. These data demonstrate that maltodextrin acquisition is likely to be a key factor in the ability of GAS to successfully infect the oropharynx. Further investigation into carbohydrate transport and metabolism pathways may yield novel insights into GAS pathogenesis.

* Corresponding author. Mailing address: Center for Molecular and Translational Human Infectious Diseases Research, The Methodist Hospital Research Institute, Houston, TX 77025. Phone: (713) 441-5890. Fax: (713) 790-6460. E-mail: jmmusser{at}tmh.tmc.edu.

Editor: D. L. Burns

Infection and Immunity, August 2006, p. 4605-4614, Vol. 74, No. 8
0019-9567/06/$08.00+0     doi:10.1128/IAI.00477-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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