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Infection and Immunity, August 2006, p. 4900-4909, Vol. 74, No. 8
0019-9567/06/$08.00+0     doi:10.1128/IAI.00412-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The BarA-UvrY Two-Component System Regulates Virulence in Avian Pathogenic Escherichia coli O78:K80:H9

Christopher D. Herren,1,{dagger} Arindam Mitra,1,2,{dagger} Senthil Kumar Palaniyandi,1,{dagger} Adam Coleman,1,3 Subbiah Elankumaran,1 and Suman Mukhopadhyay1,4*

Virginia-Maryland Regional College of Veterinary Medicine,1 Department of Animal and Avian Sciences,2 Department of Biochemistry,3 Center for Biosystems Research, University of Maryland Biotechnology Institute, University of Maryland at College Park, College Park, Maryland4

Received 14 March 2006/ Returned for modification 17 April 2006/ Accepted 22 May 2006

The BarA-UvrY two-component system (TCS) in Escherichia coli is known to regulate a number of phenotypic traits. Both in vitro and in vivo assays, including the chicken embryo lethality assay, showed that this TCS regulates virulence in avian pathogenic E. coli (APEC) serotype O78:K80:H9. A number of virulence determinants, such as the abilities to adhere, invade, persist within tissues, survive within macrophages, and resist bactericidal effects of serum complement, were compromised in mutants lacking either the barA or uvrY gene. The reduced virulence was attributed to down regulation of type 1 and Pap fimbriae, reduced exopolysaccharide production, and increased susceptibility to oxidative stress. Our results indicate that BarA-UvrY regulates virulence properties in APEC and that the chicken embryo lethality assay can be used as a surrogate model to determine virulence determinants and their regulation in APEC strains.

* Corresponding author. Mailing address: Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland at College Park, 8075 Greenmead Drive, College Park, MD 20742-3711. Phone: (301) 314-6812. Fax: (301) 314-6855. E-mail: smukhopa{at}umd.edu.

Editor: F. C. Fang

{dagger} These authors made equal contributions to this study.

Infection and Immunity, August 2006, p. 4900-4909, Vol. 74, No. 8
0019-9567/06/$08.00+0     doi:10.1128/IAI.00412-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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