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Infection and Immunity, August 2006, p. 4945-4949, Vol. 74, No. 8
0019-9567/06/$08.00+0     doi:10.1128/IAI.00584-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Swapping of Periplasmic Domains between Brucella suis VirB8 and a pSB102 VirB8 Homologue Allows Heterologous Complementation

Gilles Patey,1 Zhong Qi,1 Gisele Bourg,1 Christian Baron,2 and David O'Callaghan1*

Institut National de la Santé et de la Recherche Médicale U431, UFR Médecine, CS83021, Avenue Kennedy, 30908 Nîmes Cedex 02, France,1 Department of Biology, McMaster University, 1280 Main St. West, Hamilton, Ontario LS8 4K1, Canada2

Received 8 April 2006/ Returned for modification 5 May 2006/ Accepted 30 May 2006

A Brucella suis mutant with a nonpolar deletion in the virB8 gene was attenuated in a macrophage infection model. Complementation with the B. suis VirB8 protein expressed from the virB promoter restored virulence. Expression of TraJ, a VirB8 homologue from plasmid pSB102, did not restore virulence; however, virulence was partially restored by a chimeric protein containing the N terminus of the B. suis VirB8 protein and the C-terminal periplasmic domain of TraJ.

* Corresponding author. Mailing address: Institut National de la Santé et de la Recherche Médicale U431, UFR Médecine, CS83021, Avenue Kennedy, 30908 Nîmes Cedex 02, France. Phone: 00 33 4 66 02 81 46. Fax: 00 33 4 66 02 81 48. E-mail: david.ocallaghan{at}univ-montp1.fr.

Editor: D. L. Burns

Infection and Immunity, August 2006, p. 4945-4949, Vol. 74, No. 8
0019-9567/06/$08.00+0     doi:10.1128/IAI.00584-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Bourg, G., Sube, R., O'Callaghan, D., Patey, G. (2009). Interactions between Brucella suis VirB8 and Its Homolog TraJ from the Plasmid pSB102 Underline the Dynamic Nature of Type IV Secretion Systems. J. Bacteriol. 191: 2985-2992 [Abstract] [Full Text]  
  • den Hartigh, A. B., Rolan, H. G., de Jong, M. F., Tsolis, R. M. (2008). VirB3 to VirB6 and VirB8 to VirB11, but Not VirB7, Are Essential for Mediating Persistence of Brucella in the Reticuloendothelial System. J. Bacteriol. 190: 4427-4436 [Abstract] [Full Text]  


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