This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wiles, S.
Right arrow Articles by Frankel, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wiles, S.
Right arrow Articles by Frankel, G.

 Previous Article  |  Next Article 

Infection and Immunity, September 2006, p. 5391-5396, Vol. 74, No. 9
0019-9567/06/$08.00+0     doi:10.1128/IAI.00848-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

In Vivo Bioluminescence Imaging of the Murine Pathogen Citrobacter rodentium

Siouxsie Wiles,1* Karen M. Pickard,2 Katian Peng,1 Thomas T. MacDonald,3 and Gad Frankel1*

Division of Cell and Molecular Biology, Imperial College London, London SW7 2AZ, United Kingdom,1 Division of Infection, Inflammation and Repair, University of Southampton School of Medicine, Southampton SO16 6YD, United Kingdom,2 Institute of Cell and Molecular Science, Barts and the London School of Medicine and Dentistry, London E1 2AT, United Kingdom3

Received 26 May 2006/ Returned for modification 20 June 2006/ Accepted 26 June 2006

Citrobacter rodentium is a natural mouse pathogen related to enteropathogenic and enterohemorrhagic Escherichia coli. We have previously utilized bioluminescence imaging (BLI) to determine the in vivo colonization dynamics of C. rodentium. However, due to the oxygen requirement of the bioluminescence system and the colonic localization of C. rodentium, in vivo localization studies were performed using harvested organs. Here, we report the detection of bioluminescent C. rodentium and commensal E. coli during colonization of the gastrointestinal tract in intact living animals. Bioluminescence was dependent on intact blood circulation, suggesting that the colonic environment is not anaerobic but nanaerobic. In addition, BLI revealed that C. rodentium colonizes the rectum, a site previously unreported for this pathogen.

* Corresponding author. Mailing address: Division of Cell and Molecular Biology, Faculty of Natural Sciences, Flowers Building, Imperial College London, London SW7 2AZ, United Kingdom. Phone: 44 020 7594 3070. Fax: 44 020 7594 3069. E-mail for Siouxsie Wiles: siouxsie.wiles{at}imperial.ac.uk. E-mail for Gad Frankel: g.frankel{at}imperial.ac.uk.

Editor: J. T. Barbieri

Infection and Immunity, September 2006, p. 5391-5396, Vol. 74, No. 9
0019-9567/06/$08.00+0     doi:10.1128/IAI.00848-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hardy, J., Chu, P., Contag, C. H. (2009). Foci of Listeria monocytogenes persist in the bone marrow. DMM 2: 39-46 [Abstract] [Full Text]  
  • Dennis, A., Kudo, T., Kruidenier, L., Girard, F., Crepin, V. F., MacDonald, T. T., Frankel, G., Wiles, S. (2008). The p50 Subunit of NF-{kappa}B Is Critical for In Vivo Clearance of the Noninvasive Enteric Pathogen Citrobacter rodentium. Infect. Immun. 76: 4978-4988 [Abstract] [Full Text]  
  • McBee, M. E., Zheng, P. Z., Rogers, A. B., Fox, J. G., Schauer, D. B. (2008). Modulation of Acute Diarrheal Illness by Persistent Bacterial Infection. Infect. Immun. 76: 4851-4858 [Abstract] [Full Text]  
  • Hemrajani, C., Marches, O., Wiles, S., Girard, F., Dennis, A., Dziva, F., Best, A., Phillips, A. D., Berger, C. N., Mousnier, A., Crepin, V. F., Kruidenier, L., Woodward, M. J., Stevens, M. P., La Ragione, R. M., MacDonald, T. T., Frankel, G. (2008). Role of NleH, a Type III Secreted Effector from Attaching and Effacing Pathogens, in Colonization of the Bovine, Ovine, and Murine Gut. Infect. Immun. 76: 4804-4813 [Abstract] [Full Text]  
  • Bergstrom, K. S. B., Guttman, J. A., Rumi, M., Ma, C., Bouzari, S., Khan, M. A., Gibson, D. L., Vogl, A. W., Vallance, B. A. (2008). Modulation of Intestinal Goblet Cell Function during Infection by an Attaching and Effacing Bacterial Pathogen. Infect. Immun. 76: 796-811 [Abstract] [Full Text]  
  • Riedel, C. U., Casey, P. G., Mulcahy, H., O'Gara, F., Gahan, C. G. M., Hill, C. (2007). Construction of p16Slux, a Novel Vector for Improved Bioluminescent Labeling of Gram-Negative Bacteria. Appl. Environ. Microbiol. 73: 7092-7095 [Abstract] [Full Text]  
  • Lane, M. C., Alteri, C. J., Smith, S. N., Mobley, H. L. T. (2007). Expression of flagella is coincident with uropathogenic Escherichia coli ascension to the upper urinary tract. Proc. Natl. Acad. Sci. USA 104: 16669-16674 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»